Sip Dinkla1, Malou Peppelman1, Jori Van Der Raadt1, Femke Atsma2, Vera M J Novotný3, Marian G J Van Kraaij4, Irma Joosten1, Giel J C G M Bosman5. 1. Department of Laboratory Medicine - Laboratory of Medical Immunology, Radboud University Medical Centre, Nijmegen Institute for Infection Inflammation and Immunity, Nijmegen, The Netherlands. 2. Department of Donor Studies, Sanquin Research, Nijmegen, The Netherlands. 3. Department of Hematology, Radboud University Medical Centre, Nijmegen Institute for Infection Inflammation and Immunity, Nijmegen, The Netherlands. 4. Unit Medical Affairs, Sanquin Blood Bank Southeast Region, Nijmegen, The Netherlands. 5. Department of Biochemistry, Radboud University Medical Centre, Nijmegen Centre for Molecular Life Sciences, Nijmegen, The Netherlands.
Abstract
BACKGROUND: Exposure of phosphatidylserine on the outside of red blood cells contributes to recognition and removal of old and damaged cells. The fraction of phosphatidylserine-exposing red blood cells varies between donors, and increases in red blood cell concentrates during storage. The susceptibility of red blood cells to stress-induced phosphatidylserine exposure increases with storage. Phosphatidylserine exposure may, therefore, constitute a link between donor variation and the quality of red blood cell concentrates. MATERIALS AND METHODS: In order to examine the relationship between storage parameters and donor characteristics, the percentage of phosphatidylserine-exposing red blood cells was measured in red blood cell concentrates during storage and in fresh red blood cells from blood bank donors. The percentage of phosphatidylserine-exposing red blood cells was compared with red blood cell susceptibility to osmotic stress-induced phosphatidylserine exposure in vitro, with the regular red blood cell concentrate quality parameters, and with the donor characteristics age, body mass index, haemoglobin level, gender and blood group. RESULTS: Phosphatidylserine exposure varies between donors, both on red blood cells freshly isolated from the blood, and on red blood cells in red blood cell concentrates. Phosphatidylserine exposure increases with storage time, and is correlated with stress-induced phosphatidylserine exposure. Increased phosphatidylserine exposure during storage was found to be associated with haemolysis and vesicle concentration in red blood cell concentrates. The percentage of phosphatidylserine-exposing red blood cells showed a positive correlation with the plasma haemoglobin concentration of the donor. DISCUSSION: The fraction of phosphatidylserine-exposing red blood cells is a parameter of red blood cell integrity in red blood cell concentrates and may be an indicator of red blood cell survival after transfusion. Measurement of phosphatidylserine exposure may be useful in the selection of donors and red blood cell concentrates for specific groups of patients.
BACKGROUND: Exposure of phosphatidylserine on the outside of red blood cells contributes to recognition and removal of old and damaged cells. The fraction of phosphatidylserine-exposing red blood cells varies between donors, and increases in red blood cell concentrates during storage. The susceptibility of red blood cells to stress-induced phosphatidylserine exposure increases with storage. Phosphatidylserine exposure may, therefore, constitute a link between donor variation and the quality of red blood cell concentrates. MATERIALS AND METHODS: In order to examine the relationship between storage parameters and donor characteristics, the percentage of phosphatidylserine-exposing red blood cells was measured in red blood cell concentrates during storage and in fresh red blood cells from blood bank donors. The percentage of phosphatidylserine-exposing red blood cells was compared with red blood cell susceptibility to osmotic stress-induced phosphatidylserine exposure in vitro, with the regular red blood cell concentrate quality parameters, and with the donor characteristics age, body mass index, haemoglobin level, gender and blood group. RESULTS: Phosphatidylserine exposure varies between donors, both on red blood cells freshly isolated from the blood, and on red blood cells in red blood cell concentrates. Phosphatidylserine exposure increases with storage time, and is correlated with stress-induced phosphatidylserine exposure. Increased phosphatidylserine exposure during storage was found to be associated with haemolysis and vesicle concentration in red blood cell concentrates. The percentage of phosphatidylserine-exposing red blood cells showed a positive correlation with the plasma haemoglobin concentration of the donor. DISCUSSION: The fraction of phosphatidylserine-exposing red blood cells is a parameter of red blood cell integrity in red blood cell concentrates and may be an indicator of red blood cell survival after transfusion. Measurement of phosphatidylserine exposure may be useful in the selection of donors and red blood cell concentrates for specific groups of patients.
Authors: Florian Lang; Karl S Lang; Philipp A Lang; Stephan M Huber; Thomas Wieder Journal: Antioxid Redox Signal Date: 2006 Jul-Aug Impact factor: 8.401
Authors: Ulrich Salzer; Rong Zhu; Marleen Luten; Hirotaka Isobe; Vassili Pastushenko; Thomas Perkmann; Peter Hinterdorfer; Giel J C G M Bosman Journal: Transfusion Date: 2007-12-07 Impact factor: 3.157
Authors: Nicolaas J H Raat; Petra M Hilarius; Tanja Johannes; Dirk de Korte; Can Ince; Arthur J Verhoeven Journal: Transfusion Date: 2008-11-25 Impact factor: 3.157
Authors: Marleen Luten; Bregt Roerdinkholder-Stoelwinder; Nicolaas P M Schaap; Willem J de Grip; Harry J Bos; Giel J C G M Bosman Journal: Transfusion Date: 2008-05-13 Impact factor: 3.157
Authors: Beth A Bouchard; Thomas Orfeo; Hollis N Keith; Elizabeth M Lavoie; Matthew Gissel; Mark Fung; Kenneth G Mann Journal: J Trauma Acute Care Surg Date: 2018-04 Impact factor: 3.313
Authors: Anna L Peters; Boukje Beuger; Donald M Mock; John A Widness; Dirk de Korte; Nicole P Juffermans; Alexander P J Vlaar; Robin van Bruggen Journal: Transfusion Date: 2016-04-04 Impact factor: 3.157