Draft Genome Sequence of Lactobacillus plantarum Strain AY01, Isolated from the Raw Material of Fermented Goat Milk Cheese.

Lactobacillus plantarum is an important probiotic that is isolated mostly from fermented foods. Here, we report the first draft genome sequence of L. plantarum strain AY01, isolated from the raw material of fermented goat milk cheese. This bacterium, with optimum growth at 30°C, has a G+C content of 43.68%.

GENOME ANNOUNCEMENT

Fermented goat milk cheese is a unique dairy product in Yunnan Province, China. A local cranberry juice is added to fresh goat milk. After fermentation, cheese is produced. Lactobacillus plantarum strain AY01 was isolated from the raw material of fresh goat milk. L. plantarum is an important lactic acid bacterial species commonly used as a probiotic.

The nucleotide sequence of the L. plantarum strain AY01 genome was determined by using an Illumina HiSeq 2000 platform. A 300-bp paired-end library was constructed and sequenced, producing 24,033,038 pairs of 100-bp reads. One hundred twenty-seven contigs (>500 bp) with a total size of 3,319,865 bp were assembled using Velvet version 1.2.03 (1), providing 723-fold coverage. The average contig length is 26,140 bp and the maximum contig length is 326,866 bp, with the overall G+C content of the L. plantarum strain AY01 genome being 43.68%.

A total of 3,174 protein-coding sequences (CDSs) with an average length of 859 bp were identified by combining the prediction results of Glimmer 3.02 (2) and Z curve (3), occupying 82.1% of the genome. Defined biological functions were assigned to 2,434 CDSs (76.7%), and another 597 CDSs (18.8%) are homologous to sequences encoding hypothetical proteins in other organisms, while 143 CDSs (4.5%) have no database match. Among all the predicted proteins, 1,811 proteins (57.1%) were assigned to functional COG categories (4) and another 532 proteins (16.8%) were assigned as having a general function prediction only. In addition, 1,317 genes (41.5%) were involved in 153 different pathways. Those for the Embden-Meyerhof-Parnas (EMP), fatty acid biosynthesis, purine, and pyrimidine metabolism pathways were complete. Fifteen amino acids, not including methionine, valine, leucine, isoleucine, and phenylalanine, could be de novo synthesized. The genome contains 66 tRNA genes and 2 rRNA operons.

A comparative analysis of 3,174 proteomes using GenBank found significant matches to the L. plantarum genome, with 1,328 proteins (41.8%) matching L. plantarum strain WCFS1, 472 proteins (14.9%) matching L. plantarum strain JDM1, and 459 proteins (14.5%) matching L. plantarum subsp. plantarum strain ATCC 14917.

This is the first genome sequence of L. plantarum strain AY01, and its availability will provide a better-defined genetic background for future studies on milk fermentation.

Nucleotide sequence accession number.

The genome shotgun project for L. plantarum AY01 has been deposited at GenBank under the accession no. AVAI00000000.