BACKGROUND: The overall mortality rate in cases of head and neck squamous cell carcinoma (HNSCC) has not improved over the past 30 years, mostly because of the high treatment failure rate among patients with regionally metastatic disease. To better understand the pathobiologic processes leading to lymphatic metastasis development, there is an urgent need for relevant animal models. METHODS: HNSCC cell lines were implanted into the tongues of athymic nude mice. Histology, immunohistochemistry, and ex vivo 2-photon microscopy were used to evaluate tumor progress and spread. RESULTS: Orthotopic xenografts of different HNSCC cell lines produced distinct patterns of survival, tumor histology, disease progression rate, and lymph node metastasis development. Remarkably, all injected cell types reached the lymph nodes within 24 hours after injection, but not all developed metastasis. CONCLUSION: This orthotopic xenograft model closely mimics several characteristics of human cancer and could be extremely valuable for translational studies focusing on lymphatic metastasis development and pathobiology.
BACKGROUND: The overall mortality rate in cases of head and neck squamous cell carcinoma (HNSCC) has not improved over the past 30 years, mostly because of the high treatment failure rate among patients with regionally metastatic disease. To better understand the pathobiologic processes leading to lymphatic metastasis development, there is an urgent need for relevant animal models. METHODS: HNSCC cell lines were implanted into the tongues of athymic nude mice. Histology, immunohistochemistry, and ex vivo 2-photon microscopy were used to evaluate tumor progress and spread. RESULTS: Orthotopic xenografts of different HNSCC cell lines produced distinct patterns of survival, tumor histology, disease progression rate, and lymph node metastasis development. Remarkably, all injected cell types reached the lymph nodes within 24 hours after injection, but not all developed metastasis. CONCLUSION: This orthotopic xenograft model closely mimics several characteristics of humancancer and could be extremely valuable for translational studies focusing on lymphatic metastasis development and pathobiology.
Authors: Tohru Hoshida; Naohide Isaka; Jeroen Hagendoorn; Emmanuelle di Tomaso; Yen-Lin Chen; Bronislaw Pytowski; Dai Fukumura; Timothy P Padera; Rakesh K Jain Journal: Cancer Res Date: 2006-08-15 Impact factor: 12.701
Authors: Joseph A Knowles; Blake Golden; Li Yan; William R Carroll; Emily E Helman; Eben L Rosenthal Journal: Laryngoscope Date: 2011-11 Impact factor: 3.325
Authors: Jonathan H Law; Amy S Whigham; Pamela S Wirth; Dan Liu; Michelle Q Pham; Sangeetha Vadivelu; Kellye C Kirkbride; Brandee T Brown; Brian B Burkey; Robert J Sinard; James L Netterville; Wendell G Yarbrough Journal: Laryngoscope Date: 2009-12 Impact factor: 3.325
Authors: Tammara L Watts; Ruwen Cui; Peter Szaniszlo; Vicente A Resto; Don W Powell; Irina V Pinchuk Journal: J Transl Med Date: 2016-12-08 Impact factor: 5.531
Authors: Jia Wang; Ruwen Cui; Cecila G Clement; Ranjana Nawgiri; Don W Powell; Irina V Pinchuk; Tammara L Watts Journal: Front Oncol Date: 2020-04-28 Impact factor: 6.244