PURPOSE: Polycystic ovary syndrome (PCOS) is a most common endocrine disorder of reproductive age women. Interleukin-6 is involved in the pathophysiological characteristics associated with polycystic ovary syndrome (PCOS). The-174 G/C IL-6 gene promoter region single nucleotide polymorphism (SNP) may influence or modulate gene function and/or transcriptional efficiency. The current study was aimed to evaluate the association between IL-6 gene -174 G/C promoter polymorphism and Polycystic Ovary Syndrome in South Indian women. METHODS: In the present study, we examined the genotypic and allele distribution among the PCOS patients (n = 104) and controls (n = 156). The genotypes of IL-6 -174 G/C SNP were analyzed by polymerase chain reaction (PCR) and sequencing analysis. The allele frequency and genotype distributions of cases and controls were analyzed using Fisher's exact test. RESULTS: The genotype frequencies observed among the 104 cases and 156 controls were G/G 66.3 % and 49.4 %, G/C 29.8 % and 46.8 %, and C/C 3.8 % and 3.8 % (OR: 1.6226, CI: 1.0574-2.4899). The G and C allele frequencies were 81.25 % and 72.8 %, and 18.75 % and 27.2 %, respectively. The genotype and allele distribution revealed significant differences between PCOS patients and controls (all P values < 0.05). CONCLUSION: Our findings showed a significant statistical association between IL-6 -174 G/C SNP and PCOS risk in South Indian women. The 'G' allele frequency influences significantly higher in PCOS patients than controls. However, the exact mechanism by which 'G' allele frequency influence PCOS patients is yet to be determined.
PURPOSE:Polycystic ovary syndrome (PCOS) is a most common endocrine disorder of reproductive age women. Interleukin-6 is involved in the pathophysiological characteristics associated with polycystic ovary syndrome (PCOS). The-174 G/C IL-6 gene promoter region single nucleotide polymorphism (SNP) may influence or modulate gene function and/or transcriptional efficiency. The current study was aimed to evaluate the association between IL-6 gene -174 G/C promoter polymorphism and Polycystic Ovary Syndrome in South Indian women. METHODS: In the present study, we examined the genotypic and allele distribution among the PCOSpatients (n = 104) and controls (n = 156). The genotypes of IL-6-174 G/C SNP were analyzed by polymerase chain reaction (PCR) and sequencing analysis. The allele frequency and genotype distributions of cases and controls were analyzed using Fisher's exact test. RESULTS: The genotype frequencies observed among the 104 cases and 156 controls were G/G 66.3 % and 49.4 %, G/C 29.8 % and 46.8 %, and C/C 3.8 % and 3.8 % (OR: 1.6226, CI: 1.0574-2.4899). The G and C allele frequencies were 81.25 % and 72.8 %, and 18.75 % and 27.2 %, respectively. The genotype and allele distribution revealed significant differences between PCOSpatients and controls (all P values < 0.05). CONCLUSION: Our findings showed a significant statistical association between IL-6-174 G/C SNP and PCOS risk in South Indian women. The 'G' allele frequency influences significantly higher in PCOSpatients than controls. However, the exact mechanism by which 'G' allele frequency influence PCOSpatients is yet to be determined.
Authors: J F Tseng; I P Ryan; T D Milam; J T Murai; E D Schriock; D V Landers; R N Taylor Journal: J Clin Endocrinol Metab Date: 1996-03 Impact factor: 5.958
Authors: Eun Youn Roh; Jong Hyun Yoon; Eun Young Song; Jin Ju Kim; Kyu Ri Hwang; Soo Hyun Seo; Sue Shin Journal: J Assist Reprod Genet Date: 2016-10-29 Impact factor: 3.412
Authors: Surya Prakash Bhatt; Randeep Guleria; Naval K Vikram; S Vivekanandhan; Yogendra Singh; A K Gupta Journal: PLoS One Date: 2018-07-12 Impact factor: 3.240