OBJECTIVE: Nitrinergic control is important in meal-induced satiety. The aim of this study was to assess functional polymorphisms in nitric oxide synthase (NOS) genes in the susceptibility to functional dyspepsia (FD). METHODS: Genomic DNA from 89 patients with FD and 180 healthy subjects matched for age and gender were typed for the gene of neuronal NOS (nNOS, rs2682826), inducible NOS (iNOS, rs2297518) and a variable number tandem repeat in intron 4 of endothelial NOS (eNOS). Patients ingested 500 mL of Ensure(®) during a 20 min period and dyspeptic symptoms were scored. RESULTS: Genotype frequencies of eNOS and iNOS were not significantly different between FD patients and controls. The frequency of the T allele in nNOS was significantly higher in FD patients compared to the controls (49 vs. 16 %; odds ratio 5.01; 95 % confidence interval 2.83-9.01; p < 0.05). Patients with the T allele in the nNOS polymorphism reported a higher satiation score than those with the CC genotype during the nutrition drink test (median 179 vs. 117; p < 0.05). CONCLUSION: The nNOS gene polymorphism is associated with susceptibility to FD and influences satiation in FD patients. Our data support the importance of NOS gene polymorphisms in the pathogenesis of FD.
OBJECTIVE: Nitrinergic control is important in meal-induced satiety. The aim of this study was to assess functional polymorphisms in nitric oxide synthase (NOS) genes in the susceptibility to functional dyspepsia (FD). METHODS: Genomic DNA from 89 patients with FD and 180 healthy subjects matched for age and gender were typed for the gene of neuronal NOS (nNOS, rs2682826), inducible NOS (iNOS, rs2297518) and a variable number tandem repeat in intron 4 of endothelial NOS (eNOS). Patients ingested 500 mL of Ensure(®) during a 20 min period and dyspeptic symptoms were scored. RESULTS: Genotype frequencies of eNOS and iNOS were not significantly different between FDpatients and controls. The frequency of the T allele in nNOS was significantly higher in FDpatients compared to the controls (49 vs. 16 %; odds ratio 5.01; 95 % confidence interval 2.83-9.01; p < 0.05). Patients with the T allele in the nNOS polymorphism reported a higher satiation score than those with the CC genotype during the nutrition drink test (median 179 vs. 117; p < 0.05). CONCLUSION: The nNOS gene polymorphism is associated with susceptibility to FD and influences satiation in FDpatients. Our data support the importance of NOS gene polymorphisms in the pathogenesis of FD.
Authors: M Tonini; R De Giorgio; F De Ponti; C Sternini; V Spelta; P Dionigi; G Barbara; V Stanghellini; R Corinaldesi Journal: Br J Pharmacol Date: 2000-01 Impact factor: 8.739
Authors: Sophia S Wang; Scott Davis; James R Cerhan; Patricia Hartge; Richard K Severson; Wendy Cozen; Qing Lan; Robert Welch; Stephen J Chanock; Nathaniel Rothman Journal: Carcinogenesis Date: 2006-03-16 Impact factor: 4.944
Authors: Anastasia Kourikou; George P Karamanolis; George D Dimitriadis; Konstantinos Triantafyllou Journal: World J Gastroenterol Date: 2015-07-07 Impact factor: 5.742