| Literature DB >> 24108140 |
René Ribeiro Silva1, Delana Andreza Melo Bezerra2, Jane Haruko Lima Kaiano2, Darleise de Souza Oliveira2, Rodrigo Vellasco Duarte Silvestre2, Yvone Benchimol Gabbay2, Balasubramanian Ganesh3, Joana D'Arc Pereira Mascarenhas2.
Abstract
Picobirnavirus (PBV) belongs to the family Picobirnaviridae. Picobirnaviruses contain a bisegmented dsRNA genome that is non-enveloped. A total of 85 pooled faecal samples were collected from the poultry of 37 farms from the Metropolitan Mesoregion of Belém (MMB), Pará state, Brazil. The viral RNA from each sample was analysed by PAGE and reverse transcriptase PCR (RT-PCR). For each county affected, at least one positive sample was selected, cloned and sequenced. The samples showed a positivity of 15.3 % (13/85) by PAGE and 49.4 % (42/85) by RT-PCR. Sequencing of these strains demonstrated a considerable RdRp gene heterogeneity that ranged from 56.1 to 100 % at the nucleotide level compared with prototypes of different species and water sewage, and from 50.3 to 100 % among themselves. Avian picobirnavirus (AvPBV) was detected in MMB broiler farms and showed a heterogeneous relationship with the prototypes used. This report includes what is believed to be the first gene sequencing of AvPBV in Brazilian broiler chickens.Entities:
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Year: 2013 PMID： 24108140 DOI： 10.1099/vir.0.054783-0
Source DB: PubMed Journal: J Gen Virol ISSN： 0022-1317 Impact factor: 3.891