Andres Stricker1, Jonathan Fleiner2, Michel Dard3, Pit Voss1, Sebastian Sauerbier1, Dieter D Bosshardt4,5,6. 1. Department of Oral and Maxillofacial Surgery, University Hospital of Freiburg, Freiburg, Germany. 2. Oral Imaging Center, Faculty of Medicine, Katholieke Universiteit Leuven, Leuven, Belgium. 3. Department of Periodontology and Implant Dentistry, New York University, New York, NY, USA. 4. Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, Bern, Switzerland. 5. Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland. 6. Robert K. Schenk Laboratory of Oral Histology, School of Dental Medicine, University of Bern, Bern, Switzerland.
Abstract
OBJECTIVE: To evaluate the suitability of a minipig model for the study of bone healing and osseointegration of dental implants following bone splitting and expansion of narrow ridges. MATERIAL AND METHODS: In four minipigs, the mandibular premolars and first molars were extracted together with removal of the buccal bone plate. Three months later, ridge splitting and expansion was performed with simultaneous placement of three titanium implants per quadrant. On one side of the mandible, the expanded bone gap between the implants was filled with an alloplastic biphasic calcium phosphate (BCP) material, while the gap on the other side was left unfilled. A barrier membrane was placed in half of the quadrants. After a healing period of 6 weeks, the animals were sacrificed for histological evaluation. RESULTS: In all groups, no bone fractures occurred, no implants were lost, all 24 implants were osseointegrated, and the gap created by bone splitting was filled with new bone, irrespective of whether BCP or a barrier membrane was used. Slight exposure of five implants was observed, but did not lead to implant loss. The level of the most coronal bone-to-implant contact varied without being dependent on the use of BCP or a barrier membrane. In all groups, the BCP particles were not present deep in the bone-filled gap. However, BCP particles were seen at the crestal bone margin, where they were partly integrated in the new bone. CONCLUSIONS: This new minipig model holds great promise for studying experimental ridge splitting/expansion. However, efforts must be undertaken to reduce implant exposure and buccal bone resorption.
OBJECTIVE: To evaluate the suitability of a minipig model for the study of bone healing and osseointegration of dental implants following bone splitting and expansion of narrow ridges. MATERIAL AND METHODS: In four minipigs, the mandibular premolars and first molars were extracted together with removal of the buccal bone plate. Three months later, ridge splitting and expansion was performed with simultaneous placement of three titanium implants per quadrant. On one side of the mandible, the expanded bone gap between the implants was filled with an alloplastic biphasic calcium phosphate (BCP) material, while the gap on the other side was left unfilled. A barrier membrane was placed in half of the quadrants. After a healing period of 6 weeks, the animals were sacrificed for histological evaluation. RESULTS: In all groups, no bone fractures occurred, no implants were lost, all 24 implants were osseointegrated, and the gap created by bone splitting was filled with new bone, irrespective of whether BCP or a barrier membrane was used. Slight exposure of five implants was observed, but did not lead to implant loss. The level of the most coronal bone-to-implant contact varied without being dependent on the use of BCP or a barrier membrane. In all groups, the BCP particles were not present deep in the bone-filled gap. However, BCP particles were seen at the crestal bone margin, where they were partly integrated in the new bone. CONCLUSIONS: This new minipig model holds great promise for studying experimental ridge splitting/expansion. However, efforts must be undertaken to reduce implant exposure and buccal bone resorption.
Authors: Giuliano M Corte; Johanna Plendl; Hana Hünigen; Kenneth C Richardson; Ole Gemeinhardt; Stefan M Niehues Journal: PLoS One Date: 2017-09-14 Impact factor: 3.240
Authors: Andres Stricker; Daniel Widmer; Boyko Gueorguiev; Dieter Wahl; Peter Varga; Fabian Duttenhoefer Journal: Biomed Res Int Date: 2018-10-14 Impact factor: 3.411
Authors: Fabian Duttenhoefer; Peter Varga; Dominik Jenni; Leonard Grünwald; Luisa Thiemann; Boyko Gueorguiev; Andres Stricker Journal: Biomed Res Int Date: 2020-11-19 Impact factor: 3.411