Literature DB >> 24102865

Antibodies are forever: a study using 12-26-year-old expired antibodies.

Maria C Argentieri1, Daniela Pilla, Alice Vanzati, Silvia Lonardi, Fabio Facchetti, Claudio Doglioni, Carlo Parravicini, Giorgio Cattoretti.   

Abstract

AIMS: The aim of this study was to investigate whether the shelf-life of diagnostic antibodies is longer than the expiry date on the label. METHODS AND
RESULTS: Four independent laboratories tested a small number of diagnostic antibodies kept at +4°C for 12-26 years, and found them to work perfectly on routine histology sections.
CONCLUSIONS: Diagnostic antibodies may have a workable half-life in excess of 10 years, and the emphasis on performance should shift to the preservation of antigenic targets in the tissue.
© 2013 John Wiley & Sons Ltd.

Keywords:  antibody; expiry date; immunohistochemistry

Mesh:

Substances:

Year:  2013        PMID: 24102865     DOI: 10.1111/his.12225

Source DB:  PubMed          Journal:  Histopathology        ISSN: 0309-0167            Impact factor:   5.087


  8 in total

1.  Elution of High-affinity (>10-9 KD) Antibodies from Tissue Sections: Clues to the Molecular Mechanism and Use in Sequential Immunostaining.

Authors:  Rossella Gendusa; Carla Rossana Scalia; Serena Buscone; Giorgio Cattoretti
Journal:  J Histochem Cytochem       Date:  2014-05-02       Impact factor: 2.479

Review 2.  An overview of technical considerations for Western blotting applications to physiological research.

Authors:  J J Bass; D J Wilkinson; D Rankin; B E Phillips; N J Szewczyk; K Smith; P J Atherton
Journal:  Scand J Med Sci Sports       Date:  2016-06-05       Impact factor: 4.221

3.  Multipanel assay of 17 tumor-associated antibodies for serological detection of stage 0/I breast cancer.

Authors:  Makoto Sumazaki; Hideaki Ogata; Yoshihiro Nabeya; Akiko Kuwajima; Takaki Hiwasa; Hideaki Shimada
Journal:  Cancer Sci       Date:  2021-03-16       Impact factor: 6.716

4.  Rapid diagnostic tests for the detection of recent dengue infections: An evaluation of six kits on clinical specimens.

Authors:  Kok-Siang Yow; Joel Aik; Eugene Yong-Meng Tan; Lee-Ching Ng; Yee-Ling Lai
Journal:  PLoS One       Date:  2021-04-01       Impact factor: 3.240

5.  Maximising the Use of Scarce qPCR Master Mixes.

Authors:  Stephen Bustin; Claire Bustin; Sara Kirvell; Tania Nolan; Reinhold Mueller; Gregory Shipley
Journal:  Int J Mol Sci       Date:  2022-07-30       Impact factor: 6.208

6.  Increasing Prevalence of Antinuclear Antibodies in the United States.

Authors:  Gregg E Dinse; Christine G Parks; Clarice R Weinberg; Caroll A Co; Jesse Wilkerson; Darryl C Zeldin; Edward K L Chan; Frederick W Miller
Journal:  Arthritis Rheumatol       Date:  2020-04-30       Impact factor: 15.483

7.  Indices of immune function used by ecologists are mostly unaffected by repeated freeze-thaw cycles and methodological deviations.

Authors:  Arne Hegemann; Sara Pardal; Kevin D Matson
Journal:  Front Zool       Date:  2017-09-01       Impact factor: 3.172

8.  Stability of Zika Virus Antibodies in Specimens from a Retrospective Serological Study.

Authors:  R Tedjo Sasmono; Edison Johar; Benediktus Yohan; Chairin Nisa Ma'roef; Amin Soebandrio; Khin Sa Myint; Paul Pronyk; Sri Rezeki Hadinegoro; Elizabeth Jane Soepardi; Alain Bouckenooghe; William Hawley; Ronald Rosenberg; Ann M Powers
Journal:  Am J Trop Med Hyg       Date:  2021-07-26       Impact factor: 2.345

  8 in total

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