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Literature DB >> 24101248

A fast and simple approach to the quantitative evaluation of fibrinogen coagulation.

Weixuan Chen¹, Danzhu Wang, Nanting Ni, Minyong Li, Yidan Liu, Binghe Wang.

Abstract

Fibrinogen is essential in the intrinsic and extrinsic blood coagulation process. Inhibition of fibrinogen aggregation could lead to anticoagulation effects. The availability of methods for easy quantitative evaluation of the coagulation process is critical to studying coagulation and its inhibition. A commonly used method is UV-Vis absorbance (405 nm) detection by a micro-plate reader. However, because of the heterogeneous nature of the resulting mixture in a coagulation process, transmission-based optical measurements give large variations. Herein, a very simple and easy method is developed for the quantitative measurements of the coagulation process. The method was validated using three known thrombin inhibitors: 4-(2-aminoethyl) benzenesulfonyl fluoride (IC50: 0.01 mM), p-amidinophenyl methanesulfonyl fluoride (IC50: 0.18 mM) and PMSF (IC50: 0.23 mM).

Entities: Chemical Disease Gene Species

Mesh：

Substances：
Fibrinogen

Year: 2013 PMID： 24101248 PMCID： PMC3947019 DOI： 10.1007/s10529-013-1365-5

Source DB: PubMed Journal: Biotechnol Lett ISSN： 0141-5492 Impact factor: 2.461

15 in total

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1. A Strategy for Rapid Discovery of Marker Peptides Associated with Fibrinolytic Efficacy of Pheretima aspergillum Based on Bioinformatics Combined with Parallel Reaction Monitoring.

Authors: Ting-Ting Feng; Jing-Xian Zhang; Yong-Peng Zhang; Jian Sun; Hong Yu; Xiang Tao; Xiu-Hong Mao; Qing Hu; Shen Ji
Journal: Molecules Date: 2022-04-20 Impact factor: 4.927

1 in total

A fast and simple approach to the quantitative evaluation of fibrinogen coagulation.

1. Polymerization of rod-like macromolecular monomers studied by stopped-flow, multiangle light scattering: set-up, data processing, and application to fibrin formation.

Review 2. ImageJ for microscopy.

Review 3. Structural aspects of the fibrinogen to fibrin conversion.

4. The action of phenylmethylsulfonyl fluoride on human acetylcholinesterase, chymotyrpsin and trypsin.

5. Method for the determination of functional (clottable) fibrinogen by the new family of ACL coagulometers.

6. Reliability of the simple 20 minute whole blood clotting test (WBCT20) as an indicator of low plasma fibrinogen concentration in patients envenomed by Bothrops snakes. Butantan Institute Antivenom Study Group.

7. The binding of thrombin by fibrin.

8. Assembly of fibrin. A light scattering study.

9. Use of a 96-well microplate reader for measuring routine enzyme activities.

10. (p-Amidinophenyl)methanesulfonyl fluoride, an irreversible inhibitor of serine proteases.

1. A Strategy for Rapid Discovery of Marker Peptides Associated with Fibrinolytic Efficacy of Pheretima aspergillum Based on Bioinformatics Combined with Parallel Reaction Monitoring.