Literature DB >> 24100575

Crystallization and preliminary structural characterization of the two actin isoforms of the malaria parasite.

Saligram Prabhakar Bhargav1, Juha Vahokoski, Esa-Pekka Kumpula, Inari Kursula.   

Abstract

Malaria is a devastating disease caused by apicomplexan parasites of the genus Plasmodium that use a divergent actin-powered molecular motor for motility and invasion. Plasmodium actin differs from canonical actins in sequence, structure and function. Here, the purification, crystallization and secondary-structure analysis of the two Plasmodium actin isoforms are presented. The recombinant parasite actins were folded and could be purified to homogeneity. Plasmodium actins I and II were crystallized in complex with the gelsolin G1 domain; the crystals diffracted to resolutions of 1.19 and 2.2 Å and belonged to space groups P2₁2₁2₁ and P2₁, respectively, each with one complex in the asymmetric unit.

Entities:  

Keywords:  Plasmodium; actin; circular-dichroism spectroscopy; malaria; protein–protein complex

Mesh：

Substances：

Year:  2013        PMID： 24100575      PMCID： PMC3792683          DOI： 10.1107/S174430911302441X

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  47 in total

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Journal:  Proc Natl Acad Sci U S A       Date:  2006-03-17       Impact factor: 11.205

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Journal:  Mol Biochem Parasitol       Date:  1988-08       Impact factor: 1.759

9.  Malaria parasite actin filaments are very short.

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  4 in total

Review 1.  Towards a molecular understanding of the apicomplexan actin motor: on a road to novel targets for malaria remedies?

Authors:  Esa Pekka Kumpula; Inari Kursula
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2.  Apicomplexan actin polymerization depends on nucleation.

Authors:  Esa-Pekka Kumpula; Isa Pires; Devaki Lasiwa; Henni Piirainen; Ulrich Bergmann; Juha Vahokoski; Inari Kursula
Journal:  Sci Rep       Date:  2017-09-22       Impact factor: 4.379

3.  Atomic view into Plasmodium actin polymerization, ATP hydrolysis, and fragmentation.

Authors:  Esa-Pekka Kumpula; Andrea J Lopez; Leila Tajedin; Huijong Han; Inari Kursula
Journal:  PLoS Biol       Date:  2019-06-14       Impact factor: 8.029

4.  Structural differences explain diverse functions of Plasmodium actins.

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Journal:  PLoS Pathog       Date:  2014-04-17       Impact factor: 6.823
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