Literature DB >> 2409841

Automated determination of DNA using the fluorochrome Hoechst 33258.

W Sterzel, P Bedford, G Eisenbrand.   

Abstract

An automated method for the determination of DNA content in fractions from the alkaline filter elution assay of DNA damage has been developed. DNA-containing fractions are mixed with a fluorochrome (Hoechst 33258) and the DNA concentration is measured fluorometrically in a continuous-flow system. The lower limit of detection is 0.05 micrograms DNA/ml, and the linearity range under the conditions used is 0-8 micrograms DNA/ml. The standard deviation (n = 10) was found to be +/- 0.83%. The results are compared with the manual method.

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Year:  1985        PMID： 2409841     DOI： 10.1016/0003-2697(85)90299-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  13 in total

1.  In vitro investigations on induction and reversal of cisplatin resistance in a rat ovarian tumor cell line.

Authors:  G Chen; W J Zeller
Journal:  J Cancer Res Clin Oncol       Date:  1990       Impact factor: 4.553

2.  Human effect monitoring in cases of occupational exposure to antineoplastic drugs: a method comparison.

Authors:  S Kevekordes; T W Gebel; M Hellwig; W Dames; H Dunkelberg
Journal:  Occup Environ Med       Date:  1998-03       Impact factor: 4.402

3.  Biochemical effects of methyl chloride in relation to its tumorigenicity.

Authors:  R Jäger; H Peter; W Sterzel; H M Bolt
Journal:  J Cancer Res Clin Oncol       Date:  1988       Impact factor: 4.553

4.  Cell cycle staging of individual cells by fluorescence microscopy.

Authors:  Vassilis Roukos; Gianluca Pegoraro; Ty C Voss; Tom Misteli
Journal:  Nat Protoc       Date:  2015-01-29       Impact factor: 13.491

5.  Biological activity of N-nitrosodiethanolamine and of potential metabolites which may arise after activation by alcohol dehydrogenase in Salmonella typhimurium, in mammalian cells, and in vivo.

Authors:  E Denkel; B L Pool; J R Schlehofer; G Eisenbrand
Journal:  J Cancer Res Clin Oncol       Date:  1986       Impact factor: 4.553

6.  Pig aortic endothelial-cell cyclic nucleotide phosphodiesterases. Use of phosphodiesterase inhibitors to evaluate their roles in regulating cyclic nucleotide levels in intact cells.

Authors:  J E Souness; B K Diocee; W Martin; S A Moodie
Journal:  Biochem J       Date:  1990-02-15       Impact factor: 3.857

Review 7.  DNA adducts and DNA damage by antineoplastic and carcinogenic N-nitrosocompounds.

Authors:  G Eisenbrand; N Müller; E Denkel; W Sterzel
Journal:  J Cancer Res Clin Oncol       Date:  1986       Impact factor: 4.553

8.  DNA damage and cell death induced by 1,2-dibromo-3-chloropropane (DBCP) and structural analogs in monolayer culture of rat hepatocytes: 3-aminobenzamide inhibits the toxicity of DBCP.

Authors:  J A Holme; E J Søderlund; G Brunborg; M Låg; S D Nelson; E Dybing
Journal:  Cell Biol Toxicol       Date:  1991-10       Impact factor: 6.691

9.  A comparison of the specificity of phosphatidylcholine synthesis by human fetal lung maintained in either organ or organotypic culture.

Authors:  P A Caesar; S J Wilson; C S Normand; A D Postle
Journal:  Biochem J       Date:  1988-07-15       Impact factor: 3.857

10.  Evaluation of DNA damage by alkaline elution technique after inhalation exposure of rats and mice to 1,3-butadiene.

Authors:  R R Vangala; R J Laib; H M Bolt
Journal:  Arch Toxicol       Date:  1993       Impact factor: 5.153
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