BACKGROUND: Uromodulin (Tamm-Horsfall protein) is the most abundant protein excreted in the urine under physiological conditions. It is exclusively produced in the kidney and secreted into the urine via proteolytic cleavage. The involvement of UMOD, the gene that encodes uromodulin, in rare autosomal dominant diseases, and its robust genome-wide association with the risk of chronic kidney disease suggest that the level of uromodulin in urine could represent a critical biomarker for kidney function. The structure of uromodulin is complex, with multiple disulfide bonds and typical domains of extracellular proteins. METHODS: Thus far, the conditions influencing stability and measurement of uromodulin in human urine have not been systematically investigated, giving inconsistent results. In this study, we used a robust, in-house ELISA to characterize the conditions of sampling and storage necessary to provide a faithful dosage of uromodulin in the urine. RESULTS: The levels of uromodulin in human urine were significantly affected by centrifugation and vortexing, as well as by the conditions and duration of storage. CONCLUSIONS: These results validate a simple, low-cost ELISA and document the optimal conditions of processing and storage for measuring uromodulin in human urine.
BACKGROUND:Uromodulin (Tamm-Horsfall protein) is the most abundant protein excreted in the urine under physiological conditions. It is exclusively produced in the kidney and secreted into the urine via proteolytic cleavage. The involvement of UMOD, the gene that encodes uromodulin, in rare autosomal dominant diseases, and its robust genome-wide association with the risk of chronic kidney disease suggest that the level of uromodulin in urine could represent a critical biomarker for kidney function. The structure of uromodulin is complex, with multiple disulfide bonds and typical domains of extracellular proteins. METHODS: Thus far, the conditions influencing stability and measurement of uromodulin in human urine have not been systematically investigated, giving inconsistent results. In this study, we used a robust, in-house ELISA to characterize the conditions of sampling and storage necessary to provide a faithful dosage of uromodulin in the urine. RESULTS: The levels of uromodulin in human urine were significantly affected by centrifugation and vortexing, as well as by the conditions and duration of storage. CONCLUSIONS: These results validate a simple, low-cost ELISA and document the optimal conditions of processing and storage for measuring uromodulin in human urine.
Authors: Menno Pruijm; Belen Ponte; Daniel Ackermann; Fred Paccaud; Idris Guessous; Georg Ehret; Antoinette Pechère-Bertschi; Bruno Vogt; Markus G Mohaupt; Pierre-Yves Martin; Sonia C Youhanna; Nadine Nägele; Peter Vollenweider; Gérard Waeber; Michel Burnier; Olivier Devuyst; Murielle Bochud Journal: Clin J Am Soc Nephrol Date: 2015-12-18 Impact factor: 8.237
Authors: Edward Pivin; Belen Ponte; Sophie de Seigneux; Daniel Ackermann; Idris Guessous; Georg Ehret; Antoinette Pechère-Bertschi; Eric Olinger; Markus Mohaupt; Bruno Vogt; Pierre-Yves Martin; Michel Burnier; Murielle Bochud; Olivier Devuyst; Menno Pruijm Journal: Clin J Am Soc Nephrol Date: 2018-07-27 Impact factor: 8.237
Authors: Dominik Steubl; Petra Buzkova; Pranav S Garimella; Joachim H Ix; Prasad Devarajan; Michael R Bennett; Paolo H M Chaves; Michael G Shlipak; Nisha Bansal; Mark J Sarnak Journal: Am J Kidney Dis Date: 2019-05-22 Impact factor: 8.860