D J Miric1, B M Kisic1, L D Zoric2, B M Miric3, M Mirkovic2, R Mitic4. 1. Institute of Biochemistry, Medical Faculty Pristina, Kosovska Mitrovica, Serbia. 2. Eye Disease Clinic, Medical Faculty Pristina, Kosovska Mitrovica, Serbia. 3. Department of Informatics, State University of Novi Pazar, Novi Pazar, Serbia. 4. Institute of Pharmacology, Medical Faculty Pristina, Kosovska Mitrovica, Serbia.
Abstract
PURPOSE: The impact of cataract maturity on the aqueous humor (AH) oxidant/antioxidant balance is largely controversial. This study was aimed at assessing the relationships between cataract maturity and AH lipid peroxidation markers and enzymatic antioxidants. PATIENTS AND METHODS: The concentrations of conjugated dienes (CD), lipofuscin-like fluorescent end-products (LLF), soluble proteins, as well as the activities of superoxide dismutase (SOD) and catalase (CAT) were measured in AH samples from nondiabetic patients with either immature (n=15) or mature (n=15) cataract. RESULTS: In the overall AH sample, the mean values of CD, LLF, SOD, and CAT were 0.160 ± 0.024 (OD234), 166 ± 27 RFU, 24.5 ± 7.1 U/ml, and 31.9 ± 3.9 pmol/ml, respectively. CD was positively correlated with SOD (r=0.647; P<0.001), CAT (r=-0.394; P=0.031), and LLF (r=-0.399; P=0.029). The LLF was negatively correlated with SOD (r=-0.461; P=0.010). In samples adjusted for confounding factors, differences between immature and mature cataract groups regarding SOD, CD, LLF, and total proteins were significant (P<0.05; for all variables). The multiple logistic regression analysis identified LLF (OR=4.08; P=0.038) and SOD (OR=4.99; P=0.031) as independent predictors of cataract maturity. CONCLUSIONS: These results suggest that AH lipid peroxidation markers and antioxidants may significantly depend on the cataract maturity stage.
PURPOSE: The impact of cataract maturity on the aqueous humor (AH) oxidant/antioxidant balance is largely controversial. This study was aimed at assessing the relationships between cataract maturity and AH lipid peroxidation markers and enzymatic antioxidants. PATIENTS AND METHODS: The concentrations of conjugated dienes (CD), lipofuscin-like fluorescent end-products (LLF), soluble proteins, as well as the activities of superoxide dismutase (SOD) and catalase (CAT) were measured in AH samples from nondiabeticpatients with either immature (n=15) or mature (n=15) cataract. RESULTS: In the overall AH sample, the mean values of CD, LLF, SOD, and CAT were 0.160 ± 0.024 (OD234), 166 ± 27 RFU, 24.5 ± 7.1 U/ml, and 31.9 ± 3.9 pmol/ml, respectively. CD was positively correlated with SOD (r=0.647; P<0.001), CAT (r=-0.394; P=0.031), and LLF (r=-0.399; P=0.029). The LLF was negatively correlated with SOD (r=-0.461; P=0.010). In samples adjusted for confounding factors, differences between immature and mature cataract groups regarding SOD, CD, LLF, and total proteins were significant (P<0.05; for all variables). The multiple logistic regression analysis identified LLF (OR=4.08; P=0.038) and SOD (OR=4.99; P=0.031) as independent predictors of cataract maturity. CONCLUSIONS: These results suggest that AH lipid peroxidation markers and antioxidants may significantly depend on the cataract maturity stage.
Authors: Sumanta Goswami; Nancy L Sheets; Jiri Zavadil; Bharesh K Chauhan; Erwin P Bottinger; Venkat N Reddy; Marc Kantorow; Ales Cvekl Journal: Invest Ophthalmol Vis Sci Date: 2003-05 Impact factor: 4.799
Authors: Dario C Ramirez; Sandra E Gomez-Mejiba; Jean T Corbett; Leesa J Deterding; Kenneth B Tomer; Ronald P Mason Journal: Biochem J Date: 2009-01-01 Impact factor: 3.857