Literature DB >> 24096269

Rapid sequencing of influenza A virus vRNA, cRNA and mRNA non-coding regions.

Ruixue Wang1, Yongli Xiao, Jeffery K Taubenberger.   

Abstract

Characterizing the genomic sequences of influenza A viruses is important for pathophysiological and evolutionary studies. Noncoding regions (NCR) of influenza A virus have been shown to play critical roles in replication and transcription but their sequences are infrequently determined. In this study, a method employing poly(A) addition and SMART (switching mechanism at 5' end of RNA transcript) technology is described for directly determining and discriminating both NCR ends of viral RNA (vRNA), complementary RNA (cRNA), or NCR and cap sequences from viral mRNA. This modified method may also be used to characterize the NCRs of influenza A virus samples in which the RNA has been degraded. Published by Elsevier B.V.

Entities:  

Keywords:  Influenza A virus; Noncoding regions; RACE (random amplification of cDNA ends)

Mesh：

Substances：

Year:  2013        PMID： 24096269      PMCID： PMC4149180          DOI： 10.1016/j.jviromet.2013.09.005

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


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