| Literature DB >> 24095281 |
Francis McCoy1, Rashid Darbandi, Hoi Chang Lee, Kavitha Bharatham, Tudor Moldoveanu, Christy R Grace, Keela Dodd, Wenwei Lin, Si-Ing Chen, Rajendra P Tangallapally, Manabu Kurokawa, Richard E Lee, Anang A Shelat, Taosheng Chen, Douglas R Green, Robert A Harris, Sue-Hwa Lin, Rafael A Fissore, Roger J Colbran, Leta K Nutt.
Abstract
Active metabolism regulates oocyte cell death via calcium/calmodulin-dependent protein kinase II (CaMKII)-mediated phosphorylation of caspase-2, but the link between metabolic activity and CaMKII is poorly understood. Here we identify coenzyme A (CoA) as the key metabolic signal that inhibits Xenopus laevis oocyte apoptosis by directly activating CaMKII. We found that CoA directly binds to the CaMKII regulatory domain in the absence of Ca(2+) to activate CaMKII in a calmodulin-dependent manner. Furthermore, we show that CoA inhibits apoptosis not only in X. laevis oocytes but also in Murine oocytes. These findings uncover a direct mechanism of CaMKII regulation by metabolism and further highlight the importance of metabolism in preserving oocyte viability.Entities:
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Year: 2013 PMID： 24095281 PMCID： PMC3967247 DOI： 10.1016/j.molcel.2013.08.043
Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970