| Literature DB >> 24094198 |
Akimitsu Kugimiya1, Emi Takamitsu.
Abstract
An amino acid-sensing system with absorption spectrophotometric detection was developed. To ensure specific recognition of each amino acid, aminoacyl-tRNA synthetases were employed and the concentration of NADH produced by way of several enzymatic reactions was measured. Using this detection system, from 1.5 to 55 μM of histidine and from 15 to 95 μM of lysine could be measured selectively in HEPES-KOH buffer (pH 8.0).Entities:
Keywords: Amino acid; Aminoacyl-tRNA synthetase; Biosensing; Clinical examination; Food industry; β-Nicotinamide adenine dinucleotide
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Year: 2013 PMID： 24094198 DOI： 10.1016/j.msec.2013.08.004
Source DB: PubMed Journal: Mater Sci Eng C Mater Biol Appl ISSN： 0928-4931 Impact factor: 7.328