Cellulose acetate and electroendosmosis-low agarose electrophoresis: advanced methods for the separation and quantitative determination of serum creatine kinase isoenzyme levels.

Two methods for the separation and demonstration of creatine kinase isoenzymes are described i.e. electrophoresis on cellulose acetate and on electroendosmosis-low agarose. The fluorescence of NADPH as an indicator for the creatine kinase bands was used in both methods. The methods proved to be specific, reliable and highly reproducible, and allow a rather large number of samples (12-18) to be analysed in one run within a relatively short time. The prominent advantage of the proposed methods over others is their extreme sensitivity. Both methods allow linear quantification of creatine kinase isoenzymes up to 700 U/l at 25 degrees C with a lower detection limit of 3 U/l, using a minute amount of sample (2 microliters). The diagnostic value of the methods was shown by their application to sera of patients with myocardial infarction or other diseases.