J Tao1, J Shi, Y Lu, B Dou, Z Zhou, M Gao, Z Zhu. 1. Department of Orthopedics Shanghai First People's Hospital, School of Medicine Shanghai Jiao Tong University, Shanghai, China - zhuzhenan2006@126.com.
Abstract
AIM: The goal of this study was to examine the relationship between stearoyl-CoA desaturase 1 (SCD1) and osteogenesis in bone marrow mesenchymal stem cells (BM-MSCs). METHODS: SCD1 was overexpressed in BM-MSCs using lentiviral transduction. Then, osteogenesis was induced by low glucose DMEM (10% fetal bovine serum). Alkaline phosphatase (ALP) activity assays, Western blot analyses of SCD1 and osteocalcin, and stainings for ALP and alizarin red were done to assess the extent of osteogenesis in BM-MSCs. RESULTS: ALP activity was markedly higher in the SCD1 overexpressing cells compared with cells transduced with empty vector or control (untransduced) cells at 1 and 2 weeks after osteogenic induction. By contrast, ALP activities were comparable between control cells and cells transduced with empty vector. Western blot analysis demonstrated that osteocalcin levels were higher in the SCD1 overexpressing cells, compared with cells transduced with empty vector or control cells, at 1 and 2 weeks after osteogenic induction. These findings were corroborated by stainings for ALP and alizarin red that demonstrated a more active osteogenesis in the SCD1 overexpressing cells 2 weeks after osteogenic induction. CONCLUSION: Increased levels of SCD1 enhance osteogenesis in BM-MSCs.
AIM: The goal of this study was to examine the relationship between stearoyl-CoA desaturase 1 (SCD1) and osteogenesis in bone marrow mesenchymal stem cells (BM-MSCs). METHODS:SCD1 was overexpressed in BM-MSCs using lentiviral transduction. Then, osteogenesis was induced by low glucose DMEM (10% fetal bovine serum). Alkaline phosphatase (ALP) activity assays, Western blot analyses of SCD1 and osteocalcin, and stainings for ALP and alizarin red were done to assess the extent of osteogenesis in BM-MSCs. RESULTS:ALP activity was markedly higher in the SCD1 overexpressing cells compared with cells transduced with empty vector or control (untransduced) cells at 1 and 2 weeks after osteogenic induction. By contrast, ALP activities were comparable between control cells and cells transduced with empty vector. Western blot analysis demonstrated that osteocalcin levels were higher in the SCD1 overexpressing cells, compared with cells transduced with empty vector or control cells, at 1 and 2 weeks after osteogenic induction. These findings were corroborated by stainings for ALP and alizarin red that demonstrated a more active osteogenesis in the SCD1 overexpressing cells 2 weeks after osteogenic induction. CONCLUSION: Increased levels of SCD1 enhance osteogenesis in BM-MSCs.
Authors: Stefano Pantaleoni; Massimo Luchino; Alessandro Adriani; Rinaldo Pellicano; Davide Stradella; Davide Giuseppe Ribaldone; Nicoletta Sapone; Gian Carlo Isaia; Marco Di Stefano; Marco Astegiano Journal: ScientificWorldJournal Date: 2014-10-14
Authors: Alexandros Chatgilialoglu; Martina Rossi; Francesco Alviano; Paola Poggi; Chiara Zannini; Cosetta Marchionni; Francesca Ricci; Pier Luigi Tazzari; Valentina Taglioli; Philip C Calder; Laura Bonsi Journal: Stem Cell Res Ther Date: 2017-02-07 Impact factor: 6.832