Literature DB >> 2408619

Establishment of hybridomas secreting human monoclonal antibodies against tetanus toxin and hepatitis B virus surface antigen.

Y Ichimori, K Sasano, H Itoh, S Hitotsumachi, Y Kimura, K Kaneko, M Kida, K Tsukamoto.   

Abstract

Mouse-human heterohybrids (M X H) were constructed and compared with other cell lines (human or mouse) as parental cells to obtain hybrids secreting human monoclonal antibody (MoAb). One of the M X H lines, HM-5, was far superior to the others and useful for establishing hybrids secreting human MoAb. Using HM-5 as a parental cell line, we have obtained 2 hybrids secreting human anti-tetanus toxoid MoAb with neutralizing activity and a hybrid secreting human anti-hepatitis B virus surface antigen (HBsAg) MoAb which recognizes the a-determinant of HBsAg.

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Year:  1985        PMID： 2408619     DOI： 10.1016/0006-291x(85)91397-x

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  14 in total

1.  Improvement in the antibody productivity of human-human hybridomas by transfection with Tac gene.

Authors:  K Iwamoto; Y Shintani; R Sasada; T Honjo; K Kitano
Journal:  Cytotechnology       Date:  1990-07       Impact factor: 2.058

2.  Functional expression of human and mouse low density lipoprotein receptors in hybridomas.

Authors:  Y Shintani; K Iwamoto; K Kitano
Journal:  Cytotechnology       Date:  1995-01       Impact factor: 2.058

3.  Development of human monoclonal antibodies: A review.

Authors:  T Lindl
Journal:  Cytotechnology       Date:  1996-01       Impact factor: 2.058

4.  Characteristics of toxin-neutralization by anti-tetanus human monoclonal antibodies directed against the three functional domains [A], [B] and [C] of the tetanus toxin molecule and a reliable method for evaluating the protective effects of monoclonal antibodies.

Authors:  M Matsuda; M Kamei; N Sugimoto; Y Ma; S Hashizume
Journal:  Eur J Epidemiol       Date:  1992-01       Impact factor: 8.082

5.  Efficient production of anti-(hepatitis B virus) antibodies and their neutralizing activity in chimpanzees.

Authors:  H Sawada; S Iwasa; O Nishimura; K Kitano
Journal:  Appl Microbiol Biotechnol       Date:  1995-07       Impact factor: 4.813

6.  Establishment of stable mouse/human-human hybrid cell lines producing large amounts of anti-tetanus human monoclonal antibodies with high neutralizing activity.

Authors:  M Kamei; S Hashizume; N Sugimoto; K Ozutsumi; M Matsuda
Journal:  Eur J Epidemiol       Date:  1990-12       Impact factor: 8.082

7.  Effective production of anti-tetanus toxoid and anti-HBsAg human monoclonal antibodies by serum-free culture of hybridomas.

Authors:  K Kitano; Y Ichimori; H Sawada; S Iwasa; S Sasai; K Tsukamoto
Journal:  Cytotechnology       Date:  1991-01       Impact factor: 2.058

8.  Human monoclonal antibodies specific to hepatitis B virus generated in a human/mouse radiation chimera: the Trimera system.

Authors:  R Eren; I Lubin; D Terkieltaub; O Ben-Moshe; A Zauberman; R Uhlmann; T Tzahor; S Moss; E Ilan; D Shouval; E Galun; N Daudi; H Marcus; Y Reisner; S Dagan
Journal:  Immunology       Date:  1998-02       Impact factor: 7.397

9.  Improvement in the proliferative activity of human-human hybridomas at low cell density by transfection with bFGF gene.

Authors:  K Iwamoto; Y Shintani; H Sawada; K Kitano
Journal:  Cytotechnology       Date:  1991-06       Impact factor: 2.058

10.  Comparison of culture methods for human-human hybridomas secreting anti-HBsAg human monoclonal antibodies.

Authors:  Y Shintani; Y Kohno; H Sawada; K Kitano
Journal:  Cytotechnology       Date:  1991-07       Impact factor: 2.058
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