AIM: To study the adhesion molecules CD44 and E-cadherin expression in serous ovarian cancer (OC) and their relationship with clinicopathological peculiarities of tumor process and prognosis. MATERIALS AND METHODS: The study was performed on OC samples from 72 patients with serous OC of stages I-III. Expression of CD44 and E-cadherin in tumor samples was evaluated with the use of immunohistochemical analysis. RESULTS: Immunohistochemical analysis has revealed that in 58.3% of OC patients CD44 expression was detected in more than 10% of cells with average level of 30.0В±5.6%. E-cadherin expression was present in 47.2% of tumors, and 12.2В±3.6% cells were immunopositive. CD44 and E-cadherin expression depends on degree of cytomorphological malignancy and cell differentiation. A reverse correlation between CD44 and E-cadherin expression in primary serous OC (r=-0.38, p < 0.05) has been found. Increased CD44 expression (mean index) was not observed in peritoneal metastases compared to primary tumors, but analysis of individual indexes showed increase of CD44 expression in 46.6% OC patients with metastases. The dependence between overall survival of OC patients and the molecular phenotype of tumor cells, in particular, poor prognosis for OC patients with CD44(+)/E-cadherin(-) and CD44(+)/budding(+) tumor cells phenotype, has been revealed. CONCLUSION: The results of morphological and immunohistochemical analysis has shown the increase of adhesive features of serous ovarian cancer cells what may be significant for estimation of ovarian cancer aggressiveness and development of metastatic process.
AIM: To study the adhesion molecules CD44 and E-cadherin expression in serous ovarian cancer (OC) and their relationship with clinicopathological peculiarities of tumor process and prognosis. MATERIALS AND METHODS: The study was performed on OC samples from 72 patients with serous OC of stages I-III. Expression of CD44 and E-cadherin in tumor samples was evaluated with the use of immunohistochemical analysis. RESULTS: Immunohistochemical analysis has revealed that in 58.3% of OC patientsCD44 expression was detected in more than 10% of cells with average level of 30.0В±5.6%. E-cadherin expression was present in 47.2% of tumors, and 12.2В±3.6% cells were immunopositive. CD44 and E-cadherin expression depends on degree of cytomorphological malignancy and cell differentiation. A reverse correlation between CD44 and E-cadherin expression in primary serous OC (r=-0.38, p < 0.05) has been found. Increased CD44 expression (mean index) was not observed in peritoneal metastases compared to primary tumors, but analysis of individual indexes showed increase of CD44 expression in 46.6% OC patients with metastases. The dependence between overall survival of OC patients and the molecular phenotype of tumor cells, in particular, poor prognosis for OC patients with CD44(+)/E-cadherin(-) and CD44(+)/budding(+) tumor cells phenotype, has been revealed. CONCLUSION: The results of morphological and immunohistochemical analysis has shown the increase of adhesive features of serous ovarian cancer cells what may be significant for estimation of ovarian cancer aggressiveness and development of metastatic process.
Authors: Wided Najahi-Missaoui; Nhat D Quach; Amber Jenkins; Isha Dabke; Payaningal R Somanath; Brian S Cummings Journal: Pharmacol Res Perspect Date: 2019-09-06