Lise Pedersen1, Erling B Nielsen2, Majbrit K Christensen2, Maybritt Buchwald2, Mads Nybo2. 1. Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, Odense, Denmark lise-pedersen@rsyd.dk. 2. Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, Odense, Denmark.
Abstract
BACKGROUND: Plasma viscosity (PV) is used in the investigation of hyperviscosity syndrome. We have evaluated the performance of a new bench-top free oscillation rheometer, ReoRox from Medirox, and established a new reference range. METHODS: Performance of the free oscillation rheometry (FOR) method was evaluated examining within-run and between-run imprecision, duration of measurement and operator simplicity. Furthermore, influence of storage on the PV measurements was assessed and a reference range based on plasma from 585 healthy men and women was established. RESULTS: Imprecision of the free oscillation rheometer was comparable to other viscometers and performance of the instrument was satisfying. The non-parametrical reference range established was 1.22-1.44 mPa s at 37℃ (2.5th and 97.5th percentiles). CONCLUSIONS: We have established a FOR PV reference range to support clinical decision-making. Furthermore, we found high pre-analytical stability of the samples and an analytical imprecision comparable to other PV methods.
BACKGROUND: Plasma viscosity (PV) is used in the investigation of hyperviscosity syndrome. We have evaluated the performance of a new bench-top free oscillation rheometer, ReoRox from Medirox, and established a new reference range. METHODS: Performance of the free oscillation rheometry (FOR) method was evaluated examining within-run and between-run imprecision, duration of measurement and operator simplicity. Furthermore, influence of storage on the PV measurements was assessed and a reference range based on plasma from 585 healthy men and women was established. RESULTS: Imprecision of the free oscillation rheometer was comparable to other viscometers and performance of the instrument was satisfying. The non-parametrical reference range established was 1.22-1.44 mPa s at 37℃ (2.5th and 97.5th percentiles). CONCLUSIONS: We have established a FOR PV reference range to support clinical decision-making. Furthermore, we found high pre-analytical stability of the samples and an analytical imprecision comparable to other PV methods.
Authors: Pujith R S Vijayaratnam; Caroline C O'Brien; John A Reizes; Tracie J Barber; Elazer R Edelman Journal: PLoS One Date: 2015-06-12 Impact factor: 3.240