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Literature DB >> 24079011

Rescuing recombinant proteins by sequestration into the P22 VLP.

Dustin P Patterson¹, Benjamin LaFrance, Trevor Douglas.

Abstract

Here we report the use of a self-assembling protein cage to sequester and solubilize recombinant proteins which are usually trafficked to insoluble inclusion bodies. Our results suggest that protein cages can be used as novel vehicles to rescue and produce soluble proteins that are otherwise difficult to obtain using conventional methods.

Entities: CellLine Chemical Species

Mesh：

Substances：
Recombinant Proteins

Year: 2013 PMID： 24079011 PMCID： PMC3882142 DOI： 10.1039/c3cc46517a

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.222

17 in total

1. Nanoreactors by programmed enzyme encapsulation inside the capsid of the bacteriophage P22.

Authors: Dustin P Patterson; Peter E Prevelige; Trevor Douglas
Journal: ACS Nano Date: 2012-05-31 Impact factor: 15.881

2. Engineering of SV40-based nano-capsules for delivery of heterologous proteins as fusions with the minor capsid proteins VP2/3.

Authors: Takamasa Inoue; Masa-aki Kawano; Ryou-u Takahashi; Hiroko Tsukamoto; Teruya Enomoto; Takeshi Imai; Kohsuke Kataoka; Hiroshi Handa
Journal: J Biotechnol Date: 2008-01-03 Impact factor: 3.307

3. Infinite coordination polymer nano- and microparticle structures.

Authors: Alexander M Spokoyny; Dongwoo Kim; Abdelqader Sumrein; Chad A Mirkin
Journal: Chem Soc Rev Date: 2009-02-27 Impact factor: 54.564

4. Genetically programmed in vivo packaging of protein cargo and its controlled release from bacteriophage P22.

Authors: Alison O'Neil; Courtney Reichhardt; Benjamin Johnson; Peter E Prevelige; Trevor Douglas
Journal: Angew Chem Int Ed Engl Date: 2011-06-28 Impact factor: 15.336

5. Coconfinement of fluorescent proteins: spatially enforced communication of GFP and mCherry encapsulated within the P22 capsid.

Authors: Alison O'Neil; Peter E Prevelige; Gautam Basu; Trevor Douglas
Journal: Biomacromolecules Date: 2012-11-09 Impact factor: 6.988

6. Molecular genetics of bacteriophage P22 scaffolding protein's functional domains.

Authors: Peter R Weigele; Laura Sampson; Danella Winn-Stapley; Sherwood R Casjens
Journal: J Mol Biol Date: 2005-05-13 Impact factor: 5.469

7. Structure and assembly of the capsid of bacteriophage P22.

Authors: J King; D Botstein; S Casjens; W Earnshaw; S Harrison; E Lenk
Journal: Philos Trans R Soc Lond B Biol Sci Date: 1976-11-30 Impact factor: 6.237

8. Purification and characterization of extremely thermostable beta-mannanase, beta-mannosidase, and alpha-galactosidase from the hyperthermophilic eubacterium Thermotoga neapolitana 5068.

Authors: G D Duffaud; C M McCutchen; P Leduc; K N Parker; R M Kelly
Journal: Appl Environ Microbiol Date: 1997-01 Impact factor: 4.792

9. Colorful virus-like particles: fluorescent protein packaging by the Qβ capsid.

Authors: Jin-Kyu Rhee; Marisa Hovlid; Jason D Fiedler; Steven D Brown; Florian Manzenrieder; Hiroaki Kitagishi; Corwin Nycholat; James C Paulson; M G Finn
Journal: Biomacromolecules Date: 2011-10-13 Impact factor: 6.988

10. Properties of an alpha-galactosidase, and structure of its gene galA, within an alpha-and beta-galactoside utilization gene cluster of the hyperthermophilic bacterium Thermotoga maritima.

Authors: W Liebl; B Wagner; J Schellhase
Journal: Syst Appl Microbiol Date: 1998-03 Impact factor: 4.022

8 in total

1. Sortase-Mediated Ligation as a Modular Approach for the Covalent Attachment of Proteins to the Exterior of the Bacteriophage P22 Virus-like Particle.

Authors: Dustin Patterson; Benjamin Schwarz; John Avera; Brian Western; Matthew Hicks; Paul Krugler; Matthew Terra; Masaki Uchida; Kimberly McCoy; Trevor Douglas
Journal: Bioconjug Chem Date: 2017-06-30 Impact factor: 4.774

Rescuing recombinant proteins by sequestration into the P22 VLP.

1. Nanoreactors by programmed enzyme encapsulation inside the capsid of the bacteriophage P22.

2. Engineering of SV40-based nano-capsules for delivery of heterologous proteins as fusions with the minor capsid proteins VP2/3.

3. Infinite coordination polymer nano- and microparticle structures.

4. Genetically programmed in vivo packaging of protein cargo and its controlled release from bacteriophage P22.

5. Coconfinement of fluorescent proteins: spatially enforced communication of GFP and mCherry encapsulated within the P22 capsid.

6. Molecular genetics of bacteriophage P22 scaffolding protein's functional domains.

7. Structure and assembly of the capsid of bacteriophage P22.

8. Purification and characterization of extremely thermostable beta-mannanase, beta-mannosidase, and alpha-galactosidase from the hyperthermophilic eubacterium Thermotoga neapolitana 5068.

9. Colorful virus-like particles: fluorescent protein packaging by the Qβ capsid.

10. Properties of an alpha-galactosidase, and structure of its gene galA, within an alpha-and beta-galactoside utilization gene cluster of the hyperthermophilic bacterium Thermotoga maritima.

1. Sortase-Mediated Ligation as a Modular Approach for the Covalent Attachment of Proteins to the Exterior of the Bacteriophage P22 Virus-like Particle.

Review 2. Design of virus-based nanomaterials for medicine, biotechnology, and energy.

3. Programmed Self-Assembly of an Active P22-Cas9 Nanocarrier System.

Review 4. Biomedical and Catalytic Opportunities of Virus-Like Particles in Nanotechnology.

5. Thermostable exoshells fold and stabilize recombinant proteins.

Review 6. Virus-like particles: Next-generation nanoparticles for targeted therapeutic delivery.

Review 7. Bioengineering Strategies for Protein-Based Nanoparticles.

Review 8. Harnessing physicochemical properties of virus capsids for designing enzyme confined nanocompartments.