PURPOSE: The primary objective of this study is to investigate the content of biologically active compounds producing an antioxidant effect in Plantago maxima and their influence on main mechanisms of dietary obesity development. METHODS: Biologically active compounds in P. maxima were tested using paper chromatography. In in vivo experiment, high-fat-fed Wistar rats obtained P. maxima water extract for 3 months. Morphometric parameters, weight gain, serum adipokines, and cytokines, as well as oxidative stress biomarkers in rats’ tissues were evaluated. Gut microflora was also examined. RESULTS: Plantago maxima leaves used in the experiment contained significant amount of flavonoids, iridoids, phenol carboxylic acids, and tannins and ascorbic acid. Our in vivo experiment data demonstrate that P. maxima water extract prevents excessive adiposity in a diet-induced model. P. maxima consumption reduced serum leptin (twofold), macrophage chemoattractant protein-1 (sevenfold), tumornecrosis factor-α (25%), and interleukine-6 (26%) levels. P. maxima water extract decreased adipose tissue oxidative stress biomarkers in rats fed a high-fat diet. In addition, increased bacterial growth in the diet-induced obesity model was reversed by the P. maxima extract treatment. CONCLUSION: Plantago maxima water extract possessed antiadipogenic, antidiabetic, antiinflammatory, antioxidant activity, and normalized gut microflora in a rat model of diet-induced excessive adiposity due to a high content of biologically active compounds.
PURPOSE: The primary objective of this study is to investigate the content of biologically active compounds producing an antioxidant effect in Plantago maxima and their influence on main mechanisms of dietary obesity development. METHODS: Biologically active compounds in P. maxima were tested using paper chromatography. In in vivo experiment, high-fat-fed Wistar rats obtained P. maximawater extract for 3 months. Morphometric parameters, weight gain, serum adipokines, and cytokines, as well as oxidative stress biomarkers in rats’ tissues were evaluated. Gut microflora was also examined. RESULTS:Plantago maxima leaves used in the experiment contained significant amount of flavonoids, iridoids, phenol carboxylic acids, and tannins and ascorbic acid. Our in vivo experiment data demonstrate that P. maximawater extract prevents excessive adiposity in a diet-induced model. P. maxima consumption reduced serum leptin (twofold), macrophage chemoattractant protein-1 (sevenfold), tumornecrosis factor-α (25%), and interleukine-6 (26%) levels. P. maximawater extract decreased adipose tissue oxidative stress biomarkers in rats fed a high-fat diet. In addition, increased bacterial growth in the diet-induced obesity model was reversed by the P. maxima extract treatment. CONCLUSION:Plantago maximawater extract possessed antiadipogenic, antidiabetic, antiinflammatory, antioxidant activity, and normalized gut microflora in a rat model of diet-induced excessive adiposity due to a high content of biologically active compounds.
Authors: John F Keaney; Martin G Larson; Ramachandran S Vasan; Peter W F Wilson; Izabella Lipinska; Diane Corey; Joseph M Massaro; Patrice Sutherland; Joseph A Vita; Emelia J Benjamin Journal: Arterioscler Thromb Vasc Biol Date: 2003-01-30 Impact factor: 8.311
Authors: R L Levine; D Garland; C N Oliver; A Amici; I Climent; A G Lenz; B W Ahn; S Shaltiel; E R Stadtman Journal: Methods Enzymol Date: 1990 Impact factor: 1.600
Authors: D O Okorodudu; M F Jumean; V M Montori; A Romero-Corral; V K Somers; P J Erwin; F Lopez-Jimenez Journal: Int J Obes (Lond) Date: 2010-02-02 Impact factor: 5.095
Authors: Paolo Paoli; Paolo Cirri; Anna Caselli; Francesco Ranaldi; Giulia Bruschi; Alice Santi; Guido Camici Journal: Biochim Biophys Acta Date: 2013-01-24