BACKGROUND: IL-17 is a major cytokine player in T cell mediated leukocyte associated inflammation. IL-17 is also recognized to participate in the pathophysiology of asthma. OBJECTIVE: To determine the role of IL-17 in predicting severe asthma. METHODS: We obtained serum samples from asthmatic children under the age of 5-year in three different groups of mild (n=33), moderate (n=28) and severe (n=32) persistent asthma. IL-17 serum concentrations and mRNA expression were determined by ELISA and real time PCR assays, respectively. RESULTS: Serum IL-17 concentrations were significantly higher in patients with severe asthma than the other two groups of children with mild and moderate disease (p=0.00). Mean serum IL-17 values were 142.04 pg/ml in mild group, 180.4 pg/ml in moderate group and 251.25 pg/ml in severe group. IL-17 mRNA levels were also significantly elevated in severe asthmatic patients compared to mild and moderate asthmatic children (p=0.00). CONCLUSION: Our data reveal an increase in the serum IL-17 concentration and IL-17 mRNA expression in children with severe asthma compared to those with mild and moderate forms of the disease.
BACKGROUND:IL-17 is a major cytokine player in T cell mediated leukocyte associated inflammation. IL-17 is also recognized to participate in the pathophysiology of asthma. OBJECTIVE: To determine the role of IL-17 in predicting severe asthma. METHODS: We obtained serum samples from asthmatic children under the age of 5-year in three different groups of mild (n=33), moderate (n=28) and severe (n=32) persistent asthma. IL-17 serum concentrations and mRNA expression were determined by ELISA and real time PCR assays, respectively. RESULTS: Serum IL-17 concentrations were significantly higher in patients with severe asthma than the other two groups of children with mild and moderate disease (p=0.00). Mean serum IL-17 values were 142.04 pg/ml in mild group, 180.4 pg/ml in moderate group and 251.25 pg/ml in severe group. IL-17 mRNA levels were also significantly elevated in severe asthmatic patients compared to mild and moderate asthmatic children (p=0.00). CONCLUSION: Our data reveal an increase in the serum IL-17 concentration and IL-17 mRNA expression in children with severe asthma compared to those with mild and moderate forms of the disease.
Authors: Eric B Brandt; Jocelyn M Biagini Myers; Patrick H Ryan; Gurjit K Khurana Hershey Journal: Curr Opin Pediatr Date: 2015-12 Impact factor: 2.856
Authors: Zhonghua Zhang; Jocelyn M Biagini Myers; Eric B Brandt; Patrick H Ryan; Mark Lindsey; Rachael A Mintz-Cole; Tiina Reponen; Stephen J Vesper; Frank Forde; Brandy Ruff; Stacey A Bass; Grace K LeMasters; David I Bernstein; James Lockey; Alison L Budelsky; Gurjit K Khurana Hershey Journal: J Allergy Clin Immunol Date: 2016-04-20 Impact factor: 10.793
Authors: David Lamb; Dorothy De Sousa; Karsten Quast; Katrin Fundel-Clemens; Jonas S Erjefält; Caroline Sandén; Hans Jürgen Hoffmann; Marc Kästle; Ramona Schmid; Kevin Menden; Denis Delic Journal: Respir Res Date: 2021-05-22
Authors: Hong Ji; Jocelyn M Biagini Myers; Eric B Brandt; Cole Brokamp; Patrick H Ryan; Gurjit K Khurana Hershey Journal: Allergy Asthma Clin Immunol Date: 2016-10-19 Impact factor: 3.406