Hui Ye1, Xueli Bai1, Hua Gao1, Li Li1, Chunxiao Wu1, Xinping Sun1, Chunzhi Zhang1, Yajuan Shen1, Jian Zhang2, Zhiming Lu3. 1. Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University. 2. Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University. Electronic address: slyy2008@126.com. 3. Department of Clinical Laboratory, Shandong Provincial Hospital Affiliated to Shandong University. Electronic address: luzhiming@sdu.edu.cn.
Abstract
AIMS: We tested whether urinary podocalyxin-positive element (PCX+EL) can be a marker of early stage of diabetic nephropathy (DN). METHODS: DN patients (n=68) and health controls (n=28) were enrolled in this study. Patients were classified into three groups: normoalbuminuria, microalbuminuria and macroalbuminuria. Urinary PCX+EL, serum cystatin C (Scys C) and serum creatinine (SCr) were quantified, and correlations between urinary PCX+EL and urinary albumin, Scys C and SCr were examined. The comparison of diagnosis efficiency among urinary PCX+EL, Scys C and SCr was made by receiver operating characteristic (ROC) analysis. RESULTS: Urinary PCX+EL, Scys C and SCr significantly increased in DN patients compared with controls. Urinary PCX+EL increased significantly in all three patients groups compared with controls. However, the concentration of Scys C and SCr did not increase in normoalbuminuria group. Urinary albumin, Scys C and SCr correlated with urinary PCX+EL. ROC curve analysis indicated that area under the curve (AUC) of urinary PCX+EL (0.966) is higher than that of Scys C (0.857) and SCr (0.726) for discriminating nephropathy between DN patients and controls. CONCLUSION: Urinary PCX+EL may be a noninvasive marker for the early stage of DN.
AIMS: We tested whether urinary podocalyxin-positive element (PCX+EL) can be a marker of early stage of diabetic nephropathy (DN). METHODS: DN patients (n=68) and health controls (n=28) were enrolled in this study. Patients were classified into three groups: normoalbuminuria, microalbuminuria and macroalbuminuria. Urinary PCX+EL, serum cystatin C (Scys C) and serum creatinine (SCr) were quantified, and correlations between urinary PCX+EL and urinary albumin, Scys C and SCr were examined. The comparison of diagnosis efficiency among urinary PCX+EL, Scys C and SCr was made by receiver operating characteristic (ROC) analysis. RESULTS: Urinary PCX+EL, Scys C and SCr significantly increased in DN patients compared with controls. Urinary PCX+EL increased significantly in all three patients groups compared with controls. However, the concentration of Scys C and SCr did not increase in normoalbuminuria group. Urinary albumin, Scys C and SCr correlated with urinary PCX+EL. ROC curve analysis indicated that area under the curve (AUC) of urinary PCX+EL (0.966) is higher than that of Scys C (0.857) and SCr (0.726) for discriminating nephropathy between DN patients and controls. CONCLUSION: Urinary PCX+EL may be a noninvasive marker for the early stage of DN.