Literature DB >> 2407239

Chorismate synthase. Pre-steady-state kinetics of phosphate release from 5-enolpyruvylshikimate 3-phosphate.

T R Hawkes1, T Lewis, J R Coggins, D M Mousdale, D J Lowe, R N Thorneley.   

Abstract

The pre-steady-state kinetics of phosphate formation from 5-enolpyruvylshikimate 3-phosphate catalysed by Escherichia coli chorismate synthase (EC 4.6.1.4) were studied by a rapid-acid-quench technique at 25 degrees C at pH 7.5. No pre-steady-state 'burst' or 'lag' phase was observed, showing that phosphate is released concomitant with the rate-limiting step of the enzyme. The implications of this result for the mechanism of action of chorismate synthase are discussed.

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Year:  1990        PMID： 2407239      PMCID： PMC1133717          DOI： 10.1042/bj2650899

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  7 in total

1.  An improved assay for nanomole amounts of inorganic phosphate.

Authors:  P A Lanzetta; L J Alvarez; P S Reinach; O A Candia
Journal:  Anal Biochem       Date:  1979-11-15       Impact factor: 3.365

2.  The overexpression, purification and complete amino acid sequence of chorismate synthase from Escherichia coli K12 and its comparison with the enzyme from Neurospora crassa.

Authors:  P J White; G Millar; J R Coggins
Journal:  Biochem J       Date:  1988-04-15       Impact factor: 3.857

3.  The arom multifunctional enzyme from Neurospora crassa.

Authors:  J R Coggins; M R Boocock; S Chaudhuri; J M Lambert; J Lumsden; G A Nimmo; D D Smith
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

4.  Stereochemistry of the 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase reaction and the chorismate synthetase reaction.

Authors:  H G Floss; D K Onderka; M Carroll
Journal:  J Biol Chem       Date:  1972-02-10       Impact factor: 5.157

5.  Stereochemistry of chorismic acid biosynthesis.

Authors:  R K Hill; G R Newkome
Journal:  J Am Chem Soc       Date:  1969-10-08       Impact factor: 15.419

6.  The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl-shikimic acid-3-phosphate synthase.

Authors:  H C Steinrücken; N Amrhein
Journal:  Biochem Biophys Res Commun       Date:  1980-06-30       Impact factor: 3.575

Review 7.  The use of inhibitors in the study of glycosidases.

Authors:  P Lalégerie; G Legler; J M Yon
Journal:  Biochimie       Date:  1982 Nov-Dec       Impact factor: 4.079
  7 in total
  6 in total

1.  The Shikimate Pathway: Early Steps in the Biosynthesis of Aromatic Compounds.

Authors:  K. M. Herrmann
Journal:  Plant Cell       Date:  1995-07       Impact factor: 11.277

2.  Antileishmanial Activity of Lignans, Neolignans, and Other Plant Phenols.

Authors:  Jiří Pospíšil; Daniela Konrádová; Miroslav Strnad
Journal:  Prog Chem Org Nat Prod       Date:  2021

3.  (6S)-6-fluoroshikimic acid, an antibacterial agent acting on the aromatic biosynthetic pathway.

Authors:  G M Davies; K J Barrett-Bee; D A Jude; M Lehan; W W Nichols; P E Pinder; J L Thain; W J Watkins; R G Wilson
Journal:  Antimicrob Agents Chemother       Date:  1994-02       Impact factor: 5.191

4.  Escherichia coli chorismate synthase: a deuterium kinetic-isotope effect under single-turnover and steady-state conditions shows that a flavin intermediate forms before the C-(6proR)-H bond is cleaved.

Authors:  S Bornemann; S Balasubramanian; J R Coggins; C Abell; D J Lowe; R N Thorneley
Journal:  Biochem J       Date:  1995-02-01       Impact factor: 3.857

Review 5.  Noncanonical reactions of flavoenzymes.

Authors:  Pablo Sobrado
Journal:  Int J Mol Sci       Date:  2012-11-05       Impact factor: 5.923

6.  The Mycobacterium tuberculosis Rv2540c DNA sequence encodes a bifunctional chorismate synthase.

Authors:  Fernanda Ely; José E S Nunes; Evelyn K Schroeder; Jeverson Frazzon; Mário S Palma; Diógenes S Santos; Luiz A Basso
Journal:  BMC Biochem       Date:  2008-04-29       Impact factor: 4.059
  6 in total

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