OBJECTIVES: Can the characteristics of persisters in cultures of Mycobacterium tuberculosis also be found in bacilli from the sputum of pulmonary tuberculosis patients? The objective of this study was to explore whether the ability of persisters to grow in liquid but not on solid culture media, as in 100 day static cultures, can also be found in bacilli in sputum. METHODS: Serial dilutions of homogenized sputum obtained from patients before or during the first week of treatment were inoculated into broths to estimate the probable number of organisms and onto plates to give colony counts. RESULTS: Cultures in broths grew slowly to reach a maximal count at 12 weeks of probable numbers about 10-fold higher than the colony counts on plates, which did not grow beyond the initial count at 3-4 weeks. No such excess growth in liquid medium was found with control log-phase cultures. CONCLUSIONS: About 90% of the bacilli in sputum are persisters that can grow in liquid media but not on solid plates.
OBJECTIVES: Can the characteristics of persisters in cultures of Mycobacterium tuberculosis also be found in bacilli from the sputum of pulmonary tuberculosispatients? The objective of this study was to explore whether the ability of persisters to grow in liquid but not on solid culture media, as in 100 day static cultures, can also be found in bacilli in sputum. METHODS: Serial dilutions of homogenized sputum obtained from patients before or during the first week of treatment were inoculated into broths to estimate the probable number of organisms and onto plates to give colony counts. RESULTS: Cultures in broths grew slowly to reach a maximal count at 12 weeks of probable numbers about 10-fold higher than the colony counts on plates, which did not grow beyond the initial count at 3-4 weeks. No such excess growth in liquid medium was found with control log-phase cultures. CONCLUSIONS: About 90% of the bacilli in sputum are persisters that can grow in liquid media but not on solid plates.
Entities:
Keywords:
persister life cycle; persisters in sputum; therapy speed
Authors: Obolbek Turapov; Sarah Glenn; Bavesh Kana; Vadim Makarov; Peter W Andrew; Galina V Mukamolova Journal: Am J Respir Crit Care Med Date: 2014-12-15 Impact factor: 21.405
Authors: Kohta Saito; Saurabh Mishra; Thulasi Warrier; Nico Cicchetti; Jianjie Mi; Elaina Weber; Xiuju Jiang; Julia Roberts; Alexandre Gouzy; Ellen Kaplan; Christopher D Brown; Ben Gold; Carl Nathan Journal: Sci Transl Med Date: 2021-11-24 Impact factor: 19.319
Authors: Melissa D Chengalroyen; Germar M Beukes; Bhavna G Gordhan; Elizabeth M Streicher; Gavin Churchyard; Richard Hafner; Robin Warren; Kennedy Otwombe; Neil Martinson; Bavesh D Kana Journal: Am J Respir Crit Care Med Date: 2016-12-15 Impact factor: 21.405
Authors: Isobella Honeyborne; Timothy D McHugh; Iitu Kuittinen; Anna Cichonska; Dimitrios Evangelopoulos; Katharina Ronacher; Paul D van Helden; Stephen H Gillespie; Delmiro Fernandez-Reyes; Gerhard Walzl; Juho Rousu; Philip D Butcher; Simon J Waddell Journal: BMC Med Date: 2016-04-07 Impact factor: 8.775
Authors: Carl Nathan; Daniel W Fitzgerald; Kayvan Zainabadi; Kathleen Frances Walsh; Stalz Charles Vilbrun; Laurent Daniel Mathurin; Myung Hee Lee; Kohta Saito; Saurabh Mishra; Oksana Ocheretina; Jean William Pape Journal: Antimicrob Agents Chemother Date: 2021-07-16 Impact factor: 5.938
Authors: Obolbek Turapov; Benjamin D O'Connor; Asel A Sarybaeva; Caroline Williams; Hemu Patel; Abdullaat S Kadyrov; Akpay S Sarybaev; Gerrit Woltmann; Michael R Barer; Galina V Mukamolova Journal: Antimicrob Agents Chemother Date: 2016-03-25 Impact factor: 5.191