Ki Wook Yun1, Hak Young Kim1, Hee Kuk Park2, Wonyong Kim2, In Seok Lim3. 1. Department of Pediatrics, College of Medicine, Chung-Ang University, Seoul, South Korea. 2. Department of Microbiology, College of Medicine, Chung-Ang University, Seoul, South Korea. 3. Department of Pediatrics, College of Medicine, Chung-Ang University, Seoul, South Korea. Electronic address: pedwilly@gmail.com.
Abstract
BACKGROUND/ PURPOSE: The clinical aspects of virulence genes of uropathogenic Escherichia coli (UPEC) are not fully understood. This study compared the presence of virulence genes in UPEC isolated from urinary tract infections (UTIs) and asymptomatic bacteriuria (ABU) in children. METHODS: The study included children with UTI (n = 15) or ABU (n = 49) treated at Chung-Ang University Yongsan Hospital between 2010 and 2011. The strains were acquired from each urine sample collected, and 18 major virulence genes were detected by polymerase chain reaction. Antimicrobial susceptibility of all UPEC isolates was determined. RESULTS: Sixty-four E. coli strains were isolated from the urine samples. The most commonly identified virulence gene in both groups was fimH (100.0% in the UTI group and 95.9% in the ABU group). The UTI isolates showed a higher prevalence of papEF and fyuA, and a lower prevalence of feoB than ABU isolates (p < 0.01 for all). The profile of virulence gene, fimH(+)kpsMTII(+)feoB(+) also showed a significant difference between the two groups (p < 0.01). Isolates from ABU were more resistant to most antimicrobials tested. The presence of papEF, feoB, and fyuA also correlated with the antimicrobial susceptibility of UPEC. CONCLUSION: The virulence gene repertoire was different in the UPEC of UTI and ABU. The papEF, feoB, and fyuA genes showed meaningful differences between the two groups and may have an important role in the pathogenesis of overt UTI.
BACKGROUND/ PURPOSE: The clinical aspects of virulence genes of uropathogenic Escherichia coli (UPEC) are not fully understood. This study compared the presence of virulence genes in UPEC isolated from urinary tract infections (UTIs) and asymptomatic bacteriuria (ABU) in children. METHODS: The study included children with UTI (n = 15) or ABU (n = 49) treated at Chung-Ang University Yongsan Hospital between 2010 and 2011. The strains were acquired from each urine sample collected, and 18 major virulence genes were detected by polymerase chain reaction. Antimicrobial susceptibility of all UPEC isolates was determined. RESULTS: Sixty-four E. coli strains were isolated from the urine samples. The most commonly identified virulence gene in both groups was fimH (100.0% in the UTI group and 95.9% in the ABU group). The UTI isolates showed a higher prevalence of papEF and fyuA, and a lower prevalence of feoB than ABU isolates (p < 0.01 for all). The profile of virulence gene, fimH(+)kpsMTII(+)feoB(+) also showed a significant difference between the two groups (p < 0.01). Isolates from ABU were more resistant to most antimicrobials tested. The presence of papEF, feoB, and fyuA also correlated with the antimicrobial susceptibility of UPEC. CONCLUSION: The virulence gene repertoire was different in the UPEC of UTI and ABU. The papEF, feoB, and fyuA genes showed meaningful differences between the two groups and may have an important role in the pathogenesis of overt UTI.
Authors: Dennis J Horvath; Ashay S Patel; Ahmad Mohamed; Douglas W Storm; Chandra Singh; Birong Li; Jingwen Zhang; Stephen A Koff; Venkata R Jayanthi; Kevin M Mason; Sheryl S Justice Journal: J Bacteriol Date: 2016-01-11 Impact factor: 3.490
Authors: Hadi Sedigh Ebrahim-Saraie; Niloofar Zahedian Nezhad; Hamid Heidari; Ashkan Motamedifar; Mohammad Motamedifar Journal: Oman Med J Date: 2018-05