H Yan1, S Wang, H Yu, J Zhu, C Chen. 1. National Engineering Research Center for Miniaturized Detection System, College of Life Sciences, Northwest University, Xi'an, China.
Abstract
BACKGROUND: We postulated that microRNAs (miRNAs) might be involved in hepatocellular carcinoma (HCC) targeted chemotherapy with paclitaxel. This study sought to generate a list of potential miRNA-based biomarkers and their potential targets to better understand the response to paclitaxel treatment in HCC. METHODS: Cell viability proliferation assays were conducted to test the sensitivity of the HepG2 cells to paclitaxel. The morphological changes of apoptosis were assessed with 4',6-diamidino-2-phenylindole staining. Differential expression patterns of miRNA in the HepG2 cells either treated or not treated were analyzed using miRNA microarrays. RESULTS: The array experiments have identified 54 miRNAs whose basal expression levels differed by >2-fold and p < 0.05 between the two phenotypic groups. The data were validated by a quantitative real-time PCR of 8 selected miRNAs (miR-21, miR-1274a, miR-1260, miR-1290, miR-508-5p, miR-877, miR-1246, miR-183*). The PI3K/Akt, mitogen-activated protein kinase (MAPK), TGF-β, ErbB, p53, cell cycle, mammalian target of rapamycin, and Jak-STAT signaling pathways were involved in paclitaxel-induced apoptosis. CONCLUSIONS: The manipulation of one or more of these miRNAs could be an important approach for the improved management of paclitaxel therapy.
BACKGROUND: We postulated that microRNAs (miRNAs) might be involved in hepatocellular carcinoma (HCC) targeted chemotherapy with paclitaxel. This study sought to generate a list of potential miRNA-based biomarkers and their potential targets to better understand the response to paclitaxel treatment in HCC. METHODS: Cell viability proliferation assays were conducted to test the sensitivity of the HepG2 cells to paclitaxel. The morphological changes of apoptosis were assessed with 4',6-diamidino-2-phenylindole staining. Differential expression patterns of miRNA in the HepG2 cells either treated or not treated were analyzed using miRNA microarrays. RESULTS: The array experiments have identified 54 miRNAs whose basal expression levels differed by >2-fold and p < 0.05 between the two phenotypic groups. The data were validated by a quantitative real-time PCR of 8 selected miRNAs (miR-21, miR-1274a, miR-1260, miR-1290, miR-508-5p, miR-877, miR-1246, miR-183*). The PI3K/Akt, mitogen-activated protein kinase (MAPK), TGF-β, ErbB, p53, cell cycle, mammalian target of rapamycin, and Jak-STAT signaling pathways were involved in paclitaxel-induced apoptosis. CONCLUSIONS: The manipulation of one or more of these miRNAs could be an important approach for the improved management of paclitaxel therapy.
Authors: T Ujihira; K Ikeda; T Suzuki; R Yamaga; W Sato; K Horie-Inoue; T Shigekawa; A Osaki; T Saeki; K Okamoto; S Takeda; S Inoue Journal: Sci Rep Date: 2015-01-06 Impact factor: 4.379
Authors: Erica Hlavin Bell; Simon Kirste; Jessica L Fleming; Petra Stegmaier; Vanessa Drendel; Xiaokui Mo; Stella Ling; Denise Fabian; Isabel Manring; Cordula A Jilg; Wolfgang Schultze-Seemann; Maureen McNulty; Debra L Zynger; Douglas Martin; Julia White; Martin Werner; Anca L Grosu; Arnab Chakravarti Journal: PLoS One Date: 2015-03-11 Impact factor: 3.240