Literature DB >> 24055386

A new, rapid and sensitive bioluminescence assay for drug screening on Leishmania.

Lucie Paloque1, Nicolas Vidal, Magali Casanova, Aurélien Dumètre, Pierre Verhaeghe, Daniel Parzy, Nadine Azas.   

Abstract

We validated a new method, based on luciferine/luciferase bioluminescence, for drug screening on promastigotes of different Leishmania species. Results obtained with this new, rapid, reproducible, and reliable method are in good accordance with results obtained by the conventional MTT assay. This bioluminescence assay has a lower detection limit.
© 2013.

Entities:  

Keywords:  Accuracy; Assay validation; Leishmania; Linearity; Luminescence; Screening

Mesh：

Substances：

Year:  2013        PMID： 24055386     DOI： 10.1016/j.mimet.2013.09.006

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  7 in total

Review 1.  Methods for in vitro evaluating antimicrobial activity: A review.

Authors:  Mounyr Balouiri; Moulay Sadiki; Saad Koraichi Ibnsouda
Journal:  J Pharm Anal       Date:  2015-12-02

2.  Construction of Two mCherry Plasmids (pXG-mCherry) for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis.

Authors:  Andrés Vacas; Conor Sugden; Óscar Velasco-Rodriguez; Miriam Algarabel-Olona; José Peña-Guerrero; Esther Larrea; Celia Fernández-Rubio; Paul A Nguewa
Journal:  J Parasitol Res       Date:  2017-02-13

Review 3.  In-vivo monitoring of infectious diseases in living animals using bioluminescence imaging.

Authors:  Pinar Avci; Mahdi Karimi; Magesh Sadasivam; Wanessa C Antunes-Melo; Elisa Carrasco; Michael R Hamblin
Journal:  Virulence       Date:  2017-12-08       Impact factor: 5.882

4.  Past and future of trypanosomatids high-throughput phenotypic screening.

Authors:  Rafael Ferreira Dantas; Eduardo Caio Torres-Santos; Floriano Paes Silva
Journal:  Mem Inst Oswaldo Cruz       Date:  2022-03-11       Impact factor: 2.743

5.  In Vitro Infectivity Assessment by Drug Susceptibility Comparison of Recombinant Leishmania major Expressing Enhanced Green Fluorescent Protein or EGFP-Luciferase Fused Genes with Wild-Type Parasite.

Authors:  Somayeh Sadeghi; Negar Seyed; Mohammad-Hossein Etemadzadeh; Saeid Abediankenari; Sima Rafati; Tahereh Taheri
Journal:  Korean J Parasitol       Date:  2015-08-25       Impact factor: 1.341

Review 6.  Challenges and Tools for In Vitro Leishmania Exploratory Screening in the Drug Development Process: An Updated Review.

Authors:  Anita Cohen; Nadine Azas
Journal:  Pathogens       Date:  2021-12-10

Review 7.  Antimicrobial Compounds from Microorganisms.

Authors:  Cynthia Amaning Danquah; Prince Amankwah Baffour Minkah; Isaiah Osei Duah Junior; Kofi Bonsu Amankwah; Samuel Owusu Somuah
Journal:  Antibiotics (Basel)       Date:  2022-02-22
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.