Literature DB >> 24052376

The design and application of genetically encodable biosensors based on fluorescent proteins.

Robert H Newman1, Jin Zhang.   

Abstract

To track the activity of cellular signaling molecules within the endogenous cellular environment, researchers have developed a diverse set of genetically encodable fluorescent biosensors. These sensors, which can be targeted to specific subcellular regions to monitor specific pools of a given signaling molecule in real time, rely upon conformational changes in a sensor domain to alter the photophysical properties of green fluorescent protein (GFP) family members. In this introductory chapter, we first discuss the properties of GFP family members before turning our attention to the design and application of genetically encodable fluorescent biosensors to live cell imaging.

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Year:  2014        PMID： 24052376      PMCID： PMC4109810          DOI： 10.1007/978-1-62703-622-1_1

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  57 in total

1.  Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin.

Authors:  A Miyawaki; J Llopis; R Heim; J M McCaffery; J A Adams; M Ikura; R Y Tsien
Journal:  Nature       Date:  1997-08-28       Impact factor: 49.962

Review 2.  The green fluorescent protein.

Authors:  R Y Tsien
Journal:  Annu Rev Biochem       Date:  1998       Impact factor: 23.643

3.  The molecular structure of green fluorescent protein.

Authors:  F Yang; L G Moss; G N Phillips
Journal:  Nat Biotechnol       Date:  1996-10       Impact factor: 54.908

4.  Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins.

Authors:  J Llopis; J M McCaffery; A Miyawaki; M G Farquhar; R Y Tsien
Journal:  Proc Natl Acad Sci U S A       Date:  1998-06-09       Impact factor: 11.205

5.  Green fluorescent protein as a noninvasive intracellular pH indicator.

Authors:  M Kneen; J Farinas; Y Li; A S Verkman
Journal:  Biophys J       Date:  1998-03       Impact factor: 4.033

6.  Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer.

Authors:  R Heim; R Y Tsien
Journal:  Curr Biol       Date:  1996-02-01       Impact factor: 10.834

7.  Ultra-fast excited state dynamics in green fluorescent protein: multiple states and proton transfer.

Authors:  M Chattoraj; B A King; G U Bublitz; S G Boxer
Journal:  Proc Natl Acad Sci U S A       Date:  1996-08-06       Impact factor: 11.205

8.  Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein.

Authors:  K Brejc; T K Sixma; P A Kitts; S R Kain; R Y Tsien; M Ormö; S J Remington
Journal:  Proc Natl Acad Sci U S A       Date:  1997-03-18       Impact factor: 11.205

9.  Dynamic and quantitative Ca2+ measurements using improved cameleons.

Authors:  A Miyawaki; O Griesbeck; R Heim; R Y Tsien
Journal:  Proc Natl Acad Sci U S A       Date:  1999-03-02       Impact factor: 11.205

10.  Crystal structure of the Aequorea victoria green fluorescent protein.

Authors:  M Ormö; A B Cubitt; K Kallio; L A Gross; R Y Tsien; S J Remington
Journal:  Science       Date:  1996-09-06       Impact factor: 47.728
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  5 in total

1.  Harnessing Human Microphysiology Systems as Key Experimental Models for Quantitative Systems Pharmacology.

Authors:  D Lansing Taylor; Albert Gough; Mark E Schurdak; Lawrence Vernetti; Chakra S Chennubhotla; Daniel Lefever; Fen Pei; James R Faeder; Timothy R Lezon; Andrew M Stern; Ivet Bahar
Journal:  Handb Exp Pharmacol       Date:  2019

2.  Integrated Strategies to Gain a Systems-Level View of Dynamic Signaling Networks.

Authors:  Robert H Newman; Jin Zhang
Journal:  Methods Enzymol       Date:  2017-03-07       Impact factor: 1.600

3.  Light-Up "Channel Dyes" for Haloalkane-Based Protein Labeling in Vitro and in Bacterial Cells.

Authors:  Spencer A Clark; Vijay Singh; Daniel Vega Mendoza; William Margolin; Eric T Kool
Journal:  Bioconjug Chem       Date:  2016-12-01       Impact factor: 4.774

Review 4.  Quantifying the progression of non-alcoholic fatty liver disease in human biomimetic liver microphysiology systems with fluorescent protein biosensors.

Authors:  Manush Saydmohammed; Anupma Jha; Vineet Mahajan; Dillon Gavlock; Tong Ying Shun; Richard DeBiasio; Daniel Lefever; Xiang Li; Celeste Reese; Erin E Kershaw; Vijay Yechoor; Jaideep Behari; Alejandro Soto-Gutierrez; Larry Vernetti; Andrew Stern; Albert Gough; Mark T Miedel; D Lansing Taylor
Journal:  Exp Biol Med (Maywood)       Date:  2021-05-06

Review 5.  Human biomimetic liver microphysiology systems in drug development and precision medicine.

Authors:  Albert Gough; Alejandro Soto-Gutierrez; Lawrence Vernetti; Mo R Ebrahimkhani; Andrew M Stern; D Lansing Taylor
Journal:  Nat Rev Gastroenterol Hepatol       Date:  2020-12-17       Impact factor: 73.082
  5 in total

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