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Literature DB >> 2405017

Monoclonal antibodies to Nocardia asteroides and Nocardia brasiliensis antigens.

Abstract

Nocardia asteroides and Nocardia brasiliensis whole-cell extracts were used as antigens to generate monoclonal antibodies (MAbs). Six stable hybrid cell lines secreting anti-Nocardia spp. MAbs were obtained. These were characterized by enzyme-linked immunosorbent assay, Western blot (immunoblot), and immunofluorescence assay. Although all the MAbs exhibited different degrees of cross-reactivity with N. asteroides and N. brasiliensis antigens as well as with culture-filtrate antigens from Mycobacteria spp., they have the potential for use as reagents in the purification of Nocardia antigens.

Entities: Disease Species

Mesh：

Substances：

Year: 1990 PMID： 2405017 PMCID： PMC269542 DOI： 10.1128/jcm.28.1.87-91.1990

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

16 in total

1. Continuous cultures of fused cells secreting antibody of predefined specificity.

Authors: G Köhler; C Milstein
Journal: Nature Date: 1975-08-07 Impact factor: 49.962

2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

4. Immunoblotting with monoclonal antibodies: loss of immunoreactivity with human immunoglobulins arises from polypeptide chain separation.

Authors: R Thorpe; C R Bird; M Spitz
Journal: J Immunol Methods Date: 1984-10-26 Impact factor: 2.303

5. The phyloantigenic position of nocardiae revealed by examination of cytoplasmic antigens.

Authors: J B Kwapinski; E H Kwapinski; J Dowler; G Horsman
Journal: Can J Microbiol Date: 1973-08 Impact factor: 2.419

6. Antibody response in human nocardiosis: identification of two immunodominant culture-filtrate antigens derived from Nocardia asteroides.

Authors: A M Sugar; G K Schoolnik; D A Stevens
Journal: J Infect Dis Date: 1985-05 Impact factor: 5.226

Monoclonal antibodies to Nocardia asteroides and Nocardia brasiliensis antigens.

1. Continuous cultures of fused cells secreting antibody of predefined specificity.

2. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

4. Immunoblotting with monoclonal antibodies: loss of immunoreactivity with human immunoglobulins arises from polypeptide chain separation.

5. The phyloantigenic position of nocardiae revealed by examination of cytoplasmic antigens.

6. Antibody response in human nocardiosis: identification of two immunodominant culture-filtrate antigens derived from Nocardia asteroides.

7. Microimmunodiffusion test for nocardiosis.

Review 8. Nocardiosis: a literature review and a case report of Nocardia asteroides infection.

9. Complement fixation antibody test for human nocardiosis.

10. Structural studies of the cell wall polysaccharide of Nocardia asteroides R 399.

1. Monoclonal antibodies to a specific 54-kilodalton antigen of Nocardia spp.

2. Isolation and purification of two immunodominant antigens from Nocardia brasiliensis.

3. Monoclonal antibodies to P24 and P61 immunodominant antigens from Nocardia brasiliensis.

Review 4. The medically important aerobic actinomycetes: epidemiology and microbiology.