Literature DB >> 24050148

One-step cloning and chromosomal integration of DNA.

François St-Pierre1, Lun Cui, David G Priest, Drew Endy, Ian B Dodd, Keith E Shearwin.   

Abstract

We describe "clonetegration", a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.

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Year:  2013        PMID： 24050148     DOI： 10.1021/sb400021j

Source DB:  PubMed          Journal:  ACS Synth Biol        ISSN： 2161-5063            Impact factor:   5.110


  70 in total

1.  Quantitation of the DNA tethering effect in long-range DNA looping in vivo and in vitro using the Lac and λ repressors.

Authors:  David G Priest; Lun Cui; Sandip Kumar; David D Dunlap; Ian B Dodd; Keith E Shearwin
Journal:  Proc Natl Acad Sci U S A       Date:  2013-12-16       Impact factor: 11.205

2.  Increasing Long-Chain Dicarboxylic Acid Production in Candida tropicalis by Engineering Fatty Transporters.

Authors:  Lihua Zhang; Xiang Xiu; Zirui Wang; Yanjun Jiang; Han Fan; Jing Su; Songsen Sui; Songjiang Wang; Ruiming Wang; Junlin Li; Junqing Wang; Nan Li; Jianbin Wang
Journal:  Mol Biotechnol       Date:  2021-03-30       Impact factor: 2.695

3.  Metabolic engineering of Escherichia coli to produce succinate from woody hydrolysate under anaerobic conditions.

Authors:  Fayin Zhu; Chengqiang Wang; Ka-Yiu San; George N Bennett
Journal:  J Ind Microbiol Biotechnol       Date:  2020-01-27       Impact factor: 3.346

4.  RNA-Guided Recombinase-Cas9 Fusion Targets Genomic DNA Deletion and Integration.

Authors:  Kylie Standage-Beier; Nicholas Brookhouser; Parithi Balachandran; Qi Zhang; David A Brafman; Xiao Wang
Journal:  CRISPR J       Date:  2019-08

5.  Fluorescence bimolecular complementation enables facile detection of ribosome assembly defects in Escherichia coli.

Authors:  Himanshu Sharma; Baskaran Anand
Journal:  RNA Biol       Date:  2016-07-07       Impact factor: 4.652

6.  A quantitative method for proteome reallocation using minimal regulatory interventions.

Authors:  Gustavo Lastiri-Pancardo; Jonathan S Mercado-Hernández; Juhyun Kim; José I Jiménez; José Utrilla
Journal:  Nat Chem Biol       Date:  2020-07-13       Impact factor: 15.040

7.  Asymmetric positioning of Cas1-2 complex and Integration Host Factor induced DNA bending guide the unidirectional homing of protospacer in CRISPR-Cas type I-E system.

Authors:  K N R Yoganand; R Sivathanu; Siddharth Nimkar; B Anand
Journal:  Nucleic Acids Res       Date:  2016-11-29       Impact factor: 16.971

8.  CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering.

Authors:  Phuc Leo H Vo; Carlotta Ronda; Sanne E Klompe; Ethan E Chen; Christopher Acree; Harris H Wang; Samuel H Sternberg
Journal:  Nat Biotechnol       Date:  2020-11-23       Impact factor: 54.908

9.  Entry Exclusion of Conjugative Plasmids of the IncA, IncC, and Related Untyped Incompatibility Groups.

Authors:  Malika Humbert; Kévin T Huguet; Frédéric Coulombe; Vincent Burrus
Journal:  J Bacteriol       Date:  2019-04-24       Impact factor: 3.490

10.  Development of an autonomous and bifunctional quorum-sensing circuit for metabolic flux control in engineered Escherichia coli.

Authors:  Christina V Dinh; Kristala L J Prather
Journal:  Proc Natl Acad Sci U S A       Date:  2019-12-03       Impact factor: 11.205
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