Jiajia Yu1, Xiaojun Qiu2, Xianjuan Shen3, Wei Shi3, Xinhua Wu3, Guohao Gu4, Baoli Zhu5, Shaoqing Ju6. 1. Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong Jiangsu, China. 2. Department of Radiation Oncology, Affiliated Hospital of Nantong University, Nantong Jiangsu, China. 3. Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong Jiangsu, China. 4. The First Hospital Affiliated to Soochow University, Suzhou Jiangsu, China. 5. Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, People's Republic of China. 6. Laboratory Medicine Center, Affiliated Hospital of Nantong University, Nantong Jiangsu, China Department of Radiation Oncology, Affiliated Hospital of Nantong University, Nantong Jiangsu, China Surgical Comprehensive Laboratory, Affiliated Hospital of Nantong University, Nantong Jiangsu, China Public Health School of Nantong University, Nantong Jiangsu, China jsq814@hotmail.com zhubl@jscdc.cn.
Abstract
OBJECTIVES: To explore microRNA-202 (miR-202) expression in serum of patients with multiple myeloma (MM), and investigate correlations between serum miR-202 expression and the development and prognosis of MM. DESIGN AND METHODS: RNA was extracted from serum by QIAGEN miRNeasy Mini kit. Reverse transcription was performed with specific stem-loop primers. SYBR Green I QF-PCR was applied to detect the relative expression of miR-202 in 40 MM patients and 30 healthy controls. The linearity, specificity and reproducibility were evaluated. In addition, correlations between the relative expression of serum miR-202 and the concentrations of lactic acid dehydrogenase (LDH), β2M, λ light chain and κ light chain were assessed. RESULTS: The relative expression of miR-202 in MM patients 1.503 (0.161-9.831) was significantly higher than that in healthy controls 1.000 (0.105-3.046) (P < 0.01) and was significantly correlated with serum β2M and κ light chain concentrations (r = 0.366, P = 0.0305; r = 0.358, P = 0.0348). CONCLUSIONS: The relative expression of serum miR-202 in MM patients was significantly higher than that in healthy controls, and therefore it may prove to be useful in the auxiliary diagnosis of MM.
OBJECTIVES: To explore microRNA-202 (miR-202) expression in serum of patients with multiple myeloma (MM), and investigate correlations between serum miR-202 expression and the development and prognosis of MM. DESIGN AND METHODS: RNA was extracted from serum by QIAGEN miRNeasy Mini kit. Reverse transcription was performed with specific stem-loop primers. SYBR Green I QF-PCR was applied to detect the relative expression of miR-202 in 40 MM patients and 30 healthy controls. The linearity, specificity and reproducibility were evaluated. In addition, correlations between the relative expression of serum miR-202 and the concentrations of lactic acid dehydrogenase (LDH), β2M, λ light chain and κ light chain were assessed. RESULTS: The relative expression of miR-202 in MM patients 1.503 (0.161-9.831) was significantly higher than that in healthy controls 1.000 (0.105-3.046) (P < 0.01) and was significantly correlated with serum β2M and κ light chain concentrations (r = 0.366, P = 0.0305; r = 0.358, P = 0.0348). CONCLUSIONS: The relative expression of serum miR-202 in MM patients was significantly higher than that in healthy controls, and therefore it may prove to be useful in the auxiliary diagnosis of MM.
Authors: Philip L De Jager; Yiyi Ma; Cristin McCabe; Jishu Xu; Badri N Vardarajan; Daniel Felsky; Hans-Ulrich Klein; Charles C White; Mette A Peters; Ben Lodgson; Parham Nejad; Anna Tang; Lara M Mangravite; Lei Yu; Chris Gaiteri; Sara Mostafavi; Julie A Schneider; David A Bennett Journal: Sci Data Date: 2018-08-07 Impact factor: 6.444