Literature DB >> 24048543

Detection of heteroresistant Mycobacterium tuberculosis by pyrosequencing.

Anna Engström1, Sven Hoffner, Pontus Juréen.   

Abstract

The ability of pyrosequencing to detect a resistant minority population of a heteroresistant Mycobacterium tuberculosis strain was investigated by performing a titration study. A mutant signal was noted only when the proportion of mutant DNA in the DNA target was 35 to 50%, showing that the sensitivity is significantly lower than that of phenotypic drug susceptibility test methods.

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Year:  2013        PMID： 24048543      PMCID： PMC3838067          DOI： 10.1128/JCM.01761-13

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

1.  Next-generation sequencing for cancer diagnostics: a practical perspective.

Authors:  Cliff Meldrum; Maria A Doyle; Richard W Tothill
Journal:  Clin Biochem Rev       Date:  2011-11

2.  Evaluation of the analytical performance of the Xpert MTB/RIF assay.

Authors:  Robert Blakemore; Elizabeth Story; Danica Helb; JoAnn Kop; Padmapriya Banada; Michelle R Owens; Soumitesh Chakravorty; Martin Jones; David Alland
Journal:  J Clin Microbiol       Date:  2010-05-26       Impact factor: 5.948

3.  Rapid detection of rifampin resistance in Mycobacterium tuberculosis by Pyrosequencing technology.

Authors:  Pontus Jureen; Lars Engstrand; Solveig Eriksson; Anders Alderborn; Margareta Krabbe; Sven E Hoffner
Journal:  J Clin Microbiol       Date:  2006-06       Impact factor: 5.948

4.  Rapid, high-throughput detection of rifampin resistance and heteroresistance in Mycobacterium tuberculosis by use of sloppy molecular beacon melting temperature coding.

Authors:  Soumitesh Chakravorty; Harsheel Kothari; Bola Aladegbami; Eun Jin Cho; Jong Seok Lee; Sandy S Roh; Hyunchul Kim; Hyungkyung Kwak; Eun Gae Lee; Soo Hee Hwang; Padmapriya P Banada; Hassan Safi; Laura E Via; Sang-Nae Cho; Clifton E Barry; David Alland
Journal:  J Clin Microbiol       Date:  2012-04-25       Impact factor: 5.948

5.  Multicentre laboratory validation of the colorimetric redox indicator (CRI) assay for the rapid detection of extensively drug-resistant (XDR) Mycobacterium tuberculosis.

Authors:  Anandi Martin; Fabienne Paasch; Sven Docx; Krista Fissette; Belen Imperiale; Wellman Ribón; Liliana Andrea González; Jim Werngren; Anna Engström; Girts Skenders; Pontus Juréen; Sven Hoffner; Patricia Del Portillo; Nora Morcillo; Juan Carlos Palomino
Journal:  J Antimicrob Chemother       Date:  2011-01-19       Impact factor: 5.790

6.  Mechanisms of heteroresistance to isoniazid and rifampin of Mycobacterium tuberculosis in Tashkent, Uzbekistan.

Authors:  S Hofmann-Thiel; J van Ingen; K Feldmann; L Turaev; G T Uzakova; G Murmusaeva; D van Soolingen; H Hoffmann
Journal:  Eur Respir J       Date:  2008-10-01       Impact factor: 16.671

7.  Pyrosequencing for detection of lamivudine-resistant hepatitis B virus.

Authors:  Anna Lindström; Jacob Odeberg; Jan Albert
Journal:  J Clin Microbiol       Date:  2004-10       Impact factor: 5.948

8.  Feasibility of the GenoType MTBDRsl assay for fluoroquinolone, amikacin-capreomycin, and ethambutol resistance testing of Mycobacterium tuberculosis strains and clinical specimens.

Authors:  Doris Hillemann; Sabine Rüsch-Gerdes; Elvira Richter
Journal:  J Clin Microbiol       Date:  2009-04-22       Impact factor: 5.948

9.  Evaluation of the GenoType MTBDRplus assay for rifampin and isoniazid susceptibility testing of Mycobacterium tuberculosis strains and clinical specimens.

Authors:  Doris Hillemann; Sabine Rüsch-Gerdes; Elvira Richter
Journal:  J Clin Microbiol       Date:  2007-05-30       Impact factor: 5.948

10.  Digital PCR to detect and quantify heteroresistance in drug resistant Mycobacterium tuberculosis.

Authors:  Suporn Pholwat; Suzanne Stroup; Suporn Foongladda; Eric Houpt
Journal:  PLoS One       Date:  2013-02-27       Impact factor: 3.240
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  2 in total

1.  Rapid detection of isoniazid resistance in Mycobacterium tuberculosis isolates by use of real-time-PCR-based melting curve analysis.

Authors:  Siyu Hu; Guoli Li; Hui Li; Xiaoli Liu; Jianjun Niu; Shengmao Quan; Feng Wang; Huixin Wen; Ye Xu; Qingge Li
Journal:  J Clin Microbiol       Date:  2014-03-05       Impact factor: 5.948

2.  Direct Detection of Rifampin and Isoniazid Resistance in Sputum Samples from Tuberculosis Patients by High-Resolution Melt Curve Analysis.

Authors:  Divya Anthwal; Rakesh Kumar Gupta; Manpreet Bhalla; Shinjini Bhatnagar; Jaya Sivaswami Tyagi; Sagarika Haldar
Journal:  J Clin Microbiol       Date:  2017-03-22       Impact factor: 5.948
  2 in total

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