AIM: To investigate time-dependent changes caused by temporal portal vein obstruction and subsequent reperfusion in the lobe with or without an occluded portal vein. METHODS: The portal vein (PV) of the anterior lobe of the liver of a male Wistar rat (8 wk-old) was obstructed (70%) for 12, 24, 36 and 48 h, respectively, and models were sacrificed at 48 h after reperfusion (each group: n = 10). The histological changes and the status of liver regeneration were compared between a liver biopsy performed on each lobe after temporary obstruction of the portal vein in the same rat liver, and the liver extracted at the time of sacrifice (48 h after reperfusion). RESULTS: With regard to the obstructed lobe, the liver weight/body weight ratio significantly decreased according to obstruction time. On the other hand, in the non-obstructed lobe, there were no significant differences within each group. The duration of PV occlusion did not seem to be strong enough to introduce liver weight increase. Stimulation of liver regeneration was brought about in the non-occluded lobe by 12-h occlusion, and was sustained even at 48 h after reperfusion. The obstructed lobe atrophied with the passage of time in the obstructed state. However, the proliferating-cell nuclear antigen labeling index also increased at 48 h after reperfusion, and a repair mechanism was observed. CONCLUSION: Temporary blood flow obstruction of the portal vein may become a significant trigger for liver regeneration, even with an obstruction of 12 h.
AIM: To investigate time-dependent changes caused by temporal portal vein obstruction and subsequent reperfusion in the lobe with or without an occluded portal vein. METHODS: The portal vein (PV) of the anterior lobe of the liver of a male Wistar rat (8 wk-old) was obstructed (70%) for 12, 24, 36 and 48 h, respectively, and models were sacrificed at 48 h after reperfusion (each group: n = 10). The histological changes and the status of liver regeneration were compared between a liver biopsy performed on each lobe after temporary obstruction of the portal vein in the same rat liver, and the liver extracted at the time of sacrifice (48 h after reperfusion). RESULTS: With regard to the obstructed lobe, the liver weight/body weight ratio significantly decreased according to obstruction time. On the other hand, in the non-obstructed lobe, there were no significant differences within each group. The duration of PV occlusion did not seem to be strong enough to introduce liver weight increase. Stimulation of liver regeneration was brought about in the non-occluded lobe by 12-h occlusion, and was sustained even at 48 h after reperfusion. The obstructed lobe atrophied with the passage of time in the obstructed state. However, the proliferating-cell nuclear antigen labeling index also increased at 48 h after reperfusion, and a repair mechanism was observed. CONCLUSION: Temporary blood flow obstruction of the portal vein may become a significant trigger for liver regeneration, even with an obstruction of 12 h.
Authors: H Imamura; R Shimada; M Kubota; Y Matsuyama; A Nakayama; S Miyagawa; M Makuuchi; S Kawasaki Journal: Hepatology Date: 1999-04 Impact factor: 17.425
Authors: N Kokudo; K Tada; M Seki; H Ohta; K Azekura; M Ueno; K Ohta; T Yamaguchi; T Matsubara; T Takahashi; T Nakajima; T Muto; T Ikari; A Yanagisawa; Y Kato Journal: Hepatology Date: 2001-08 Impact factor: 17.425
Authors: Günter Fürst; Jan Schulte am Esch; Ludger W Poll; Stefan B Hosch; L Benjamin Fritz; Michael Klein; Erhard Godehardt; Andreas Krieg; Britta Wecker; Volker Stoldt; Marcus Stockschläder; Claus F Eisenberger; Ulrich Mödder; Wolfram T Knoefel Journal: Radiology Date: 2007-02-20 Impact factor: 11.105
Authors: B Aussilhou; M Lesurtel; A Sauvanet; O Farges; S Dokmak; N Goasguen; A Sibert; V Vilgrain; J Belghiti Journal: J Gastrointest Surg Date: 2007-11-30 Impact factor: 3.452