Literature DB >> 2403643

Effects of methotrexate on transfected DNA stability in mammalian cells.

M G Pallavicini1, P S DeTeresa, C Rosette, J W Gray, F M Wurm.   

Abstract

The chromosomal locations, amounts, and level of expression of transfected, amplified c-myc and dihydrofolate reductase sequences were measured in cells cultured in the presence and absence of methotrexate. These studies show that the location and amount of transfected sequences, as well as the level of expression, were more variable when the cells were cultured in methotrexate.

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Year:  1990        PMID： 2403643      PMCID： PMC360766          DOI： 10.1128/mcb.10.1.401-404.1990

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  10 in total

1.  Use of fluorescence in situ hybridization to detect and monitor transfected and amplified sequences in recombinant CHO cells.

Authors:  M G Pallavicini; P S DeTeresa; F M Wurm
Journal:  Dev Biol Stand       Date:  1989

2.  Inducible overproduction of the mouse c-myc protein in mammalian cells.

Authors:  F M Wurm; K A Gwinn; R E Kingston
Journal:  Proc Natl Acad Sci U S A       Date:  1986-08       Impact factor: 11.205

3.  Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4.

Authors:  D Pinkel; J Landegent; C Collins; J Fuscoe; R Segraves; J Lucas; J Gray
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

4.  Amplification and expression of sequences cotransfected with a modular dihydrofolate reductase complementary dna gene.

Authors:  R J Kaufman; P A Sharp
Journal:  J Mol Biol       Date:  1982-08-25       Impact factor: 5.469

5.  Evolution of chromosomal regions containing transfected and amplified dihydrofolate reductase sequences.

Authors:  R J Kaufman; P A Sharp; S A Latt
Journal:  Mol Cell Biol       Date:  1983-04       Impact factor: 4.272

6.  Coamplification and coexpression of human tissue-type plasminogen activator and murine dihydrofolate reductase sequences in Chinese hamster ovary cells.

Authors:  R J Kaufman; L C Wasley; A J Spiliotes; S D Gossels; S A Latt; G R Larsen; R M Kay
Journal:  Mol Cell Biol       Date:  1985-07       Impact factor: 4.272

7.  Amplified expression constructs for human tissue-type plasminogen activator in Chinese hamster ovary cells: instability in the absence of selective pressure.

Authors:  U H Weidle; P Buckel; J Wienberg
Journal:  Gene       Date:  1988-06-30       Impact factor: 3.688

8.  Sensitive, high-resolution chromatin and chromosome mapping in situ: presence and orientation of two closely integrated copies of EBV in a lymphoma line.

Authors:  J B Lawrence; C A Villnave; R H Singer
Journal:  Cell       Date:  1988-01-15       Impact factor: 41.582

9.  Amplification of a cloned Chinese hamster dihydrofolate reductase gene after transfer into a dihydrofolate reductase-deficient cell line.

Authors:  J D Milbrandt; J C Azizkhan; J L Hamlin
Journal:  Mol Cell Biol       Date:  1983-07       Impact factor: 4.272

10.  Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.

Authors:  D Pinkel; T Straume; J W Gray
Journal:  Proc Natl Acad Sci U S A       Date:  1986-05       Impact factor: 11.205
  10 in total
  11 in total

1.  Evaluation of stable and highly productive gene amplified CHO cell line based on the location of amplified genes.

Authors:  T Yoshikawa; F Nakanishi; S Itami; D Kameoka; T Omasa; Y Katakura; M Kishimoto; K Suga
Journal:  Cytotechnology       Date:  2000-07       Impact factor: 2.058

2.  Addition of valproic acid to CHO cell fed-batch cultures improves monoclonal antibody titers.

Authors:  William C Yang; Jiuyi Lu; Ngan B Nguyen; An Zhang; Nicholas V Healy; Rashmi Kshirsagar; Thomas Ryll; Yao-Ming Huang
Journal:  Mol Biotechnol       Date:  2014-05       Impact factor: 2.695

3.  Recurring genomic structural variation leads to clonal instability and loss of productivity.

Authors:  Arpan A Bandyopadhyay; Sofie A O'Brien; Liang Zhao; Hsu-Yuan Fu; Nandita Vishwanathan; Wei-Shou Hu
Journal:  Biotechnol Bioeng       Date:  2018-10-27       Impact factor: 4.530

4.  Engraftment and long-term expression of human fetal hemopoietic stem cells in sheep following transplantation in utero.

Authors:  E D Zanjani; M G Pallavicini; J L Ascensao; A W Flake; R G Langlois; M Reitsma; F R MacKintosh; D Stutes; M R Harrison; M Tavassoli
Journal:  J Clin Invest       Date:  1992-04       Impact factor: 14.808

5.  Comparative Assessment on the Expression Level of Recombinant Human Follicle-Stimulating Hormone (FSH) in Serum-Containing Versus Protein-Free Culture Media.

Authors:  Seyedeh Hoda Jazayeri; Amir Amiri-Yekta; Hamid Gourabi; Baharak Abd Emami; Zahra Halfinezhad; Somayeh Abolghasemi; Nayeralsadat Fatemi; Abbas Daneshipour; Mohammad Hossein Ghahremani; Mohammad Hossein Sanati; Mohammad Reza Khorramizadeh
Journal:  Mol Biotechnol       Date:  2017-12       Impact factor: 2.695

6.  Identification of transgene integration loci of different highly expressing recombinant CHO cell lines by FISH.

Authors:  Christine Lattenmayer; Martina Loeschel; Willibald Steinfellner; Evelyn Trummer; Dethardt Mueller; Kornelia Schriebl; Karola Vorauer-Uhl; Hermann Katinger; Renate Kunert
Journal:  Cytotechnology       Date:  2006-11-15       Impact factor: 2.058

7.  Advances in animal cell recombinant protein production: GS-NS0 expression system.

Authors:  L M Barnes; C M Bentley; A J Dickson
Journal:  Cytotechnology       Date:  2000-02       Impact factor: 2.058

8.  Development of HEK-293 Cell Lines Constitutively Expressing Flaviviral Antigens for Use in Diagnostics.

Authors:  Jordan A Powers; Benjamin Skinner; Brent S Davis; Brad J Biggerstaff; Lucy Robb; Elizabeth Gordon; William M de Souza; Marcilio Jorge Fumagalli; Amanda E Calvert; Gwong-Jen Chang
Journal:  Microbiol Spectr       Date:  2022-05-09

9.  Fusion of the Dhfr/Mtx and IR/MAR gene amplification methods produces a rapid and efficient method for stable recombinant protein production.

Authors:  Chiemi Noguchi; Yoshio Araki; Daisuke Miki; Noriaki Shimizu
Journal:  PLoS One       Date:  2012-12-31       Impact factor: 3.240

10.  Overexpression of human alpha-galactosidase A results in its intracellular aggregation, crystallization in lysosomes, and selective secretion.

Authors:  Y A Ioannou; D F Bishop; R J Desnick
Journal:  J Cell Biol       Date:  1992-12       Impact factor: 10.539
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