| Literature DB >> 24035892 |
Yuanpeng Wang1, Jiyuan Cai, Jianghuang Lan, Zhenggui Liu, Ning He, Liang Shen, Qingbiao Li.
Abstract
This study investigated the effects of substrate composition (acetate and propionate) on synthesis of various mix of poly(hydroxybutyrate-hydroxyvalerate) (P(HB/HV)) from activated sludge, which was acclimated using a single carbon (acetate) and mixed carbons (acetate and propionate). Results of batch P(HB/HV) production tests indicated that the yield and synthesis rate of P(HB/HV) decreased as the proportion of propionate in the substrate increased. However, mixed-carbon-acclimated sludge with acetate and propionate exhibited better P(HB/HV) production performance than with acetate-acclimated sludge in terms of substrate utilization, yield of P(HB/HV) and HV fraction in P(HB/HV). The desired hydroxyvalerate (HV) fraction (0-74%) of the P(HB/HV) could be obtained based on the substrate composition and sludge acclimation. Furthermore, PCR-DGGE analysis indicated that specific species dominated the activated sludge used for P(HB/HV) production. Acidobacteria and Burkholderiales were the dominant bacterial populations and played an important role in HV synthesis.Entities:
Keywords: Activated sludge; COD; DGGE; DO; HB; HRT; HV; MLVSS; MPCC; MPCT; Microbial community; P(HB/HV); P(HB/HV) yield; PCR; PHAs; PHB; PHV; SBR; SRT; Substrate composition; TCA cycle; VSS; X; Y(P(HB/HV)); biomass; chemical oxygen demand; denaturing gradient-gel-electrophoresis; dissolved oxygen; hydraulic retention time; hydroxybutyrate; hydroxyvalerate; maximum P(HB/HV) concentration; maximum P(HB/HV) content; mixed liquor volatile suspended solid; poly(hydroxybutyrate-hydroxyvalerate); polyhydroxyalkanoates; polyhydroxybutyrate; polyhydroxyvalerate; polymerase chain reaction; q(P(HB/HV)); q(s); sequencing biological reactor; solid retention time; specific P(HB/HV) synthesis rate; specific substrate consumption; tricarboxylic acid cycle; volatile suspended solid
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Year: 2013 PMID： 24035892 DOI： 10.1016/j.biortech.2013.08.102
Source DB: PubMed Journal: Bioresour Technol ISSN： 0960-8524 Impact factor: 9.642