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Literature DB >> 24034331

RNA purification--precipitation methods.

Abstract

When working with RNA, the need often arises to concentrate a sample or purify it from various salts, nucleotides, and proteins. RNA precipitation is an easy and cost-effective method for the concentration of RNA, leaving a pellet that can be resuspended in the buffer of choice.

Keywords: Ammonium acetate; Ethanol precipitation; Lithium chloride precipitation; Precipitation methods; RNA purification; Sodium acetate

Mesh：

Substances：

Year: 2013 PMID： 24034331 DOI： 10.1016/B978-0-12-420037-1.00019-1

Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600

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Cited

22 in total

1. Novel 5'/3'RACE Method for Amplification and Determination of Single-Stranded RNAs Through Double-Stranded RNA (dsRNA) Intermediates.

Authors: Péter Pankovics; Ákos Boros; Gábor Reuter
Journal: Mol Biotechnol Date: 2015-12 Impact factor: 2.695

2. Reconstitution and analyses of RNA interactions with eukaryotic translation initiation factors and ribosomal preinitiation complexes.

Authors: Xiaozhuo Liu; Peter J Schuessler; Ansuman Sahoo; Sarah E Walker
Journal: Methods Date: 2019-03-26 Impact factor: 3.608

3. Elucidating the Burden of HIV in Tissues Using Multiplexed Immunofluorescence and In Situ Hybridization: Methods for the Single-Cell Phenotypic Characterization of Cells Harboring HIV In Situ.

Authors: Joshua J Vasquez; Rajaa Hussien; Brandon Aguilar-Rodriguez; Henrik Junger; Dejan Dobi; Timothy J Henrich; Cassandra Thanh; Erica Gibson; Louise E Hogan; Joseph McCune; Peter W Hunt; Cheryl A Stoddart; Zoltan G Laszik
Journal: J Histochem Cytochem Date: 2018-02-20 Impact factor: 2.479

4. Orchestrated translation specializes dinoflagellate metabolism three times per day.

Authors: Carl Bowazolo; Bo Song; Sonia Dorion; Mathieu Beauchemin; Samuel Chevrier; Jean Rivoal; David Morse
Journal: Proc Natl Acad Sci U S A Date: 2022-07-18 Impact factor: 12.779

5. Established Protocols for cRNA Expression and Voltage-Clamp Characterization of the P2X7 Receptor in Xenopus laevis Oocytes.

Authors: Günther Schmalzing; Fritz Markwardt
Journal: Methods Mol Biol Date: 2022

6. Swine Influenza Virus PA and Neuraminidase Gene Reassortment into Human H1N1 Influenza Virus Is Associated with an Altered Pathogenic Phenotype Linked to Increased MIP-2 Expression.

Authors: Daniel Dlugolenski; Les Jones; Elizabeth Howerth; David Wentworth; S Mark Tompkins; Ralph A Tripp
Journal: J Virol Date: 2015-03-11 Impact factor: 5.103

RNA purification--precipitation methods.

1. Novel 5'/3'RACE Method for Amplification and Determination of Single-Stranded RNAs Through Double-Stranded RNA (dsRNA) Intermediates.

2. Reconstitution and analyses of RNA interactions with eukaryotic translation initiation factors and ribosomal preinitiation complexes.

3. Elucidating the Burden of HIV in Tissues Using Multiplexed Immunofluorescence and In Situ Hybridization: Methods for the Single-Cell Phenotypic Characterization of Cells Harboring HIV In Situ.

4. Orchestrated translation specializes dinoflagellate metabolism three times per day.

5. Established Protocols for cRNA Expression and Voltage-Clamp Characterization of the P2X7 Receptor in Xenopus laevis Oocytes.

6. Swine Influenza Virus PA and Neuraminidase Gene Reassortment into Human H1N1 Influenza Virus Is Associated with an Altered Pathogenic Phenotype Linked to Increased MIP-2 Expression.

7. Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice.

8. Dengue virus activates cGAS through the release of mitochondrial DNA.

9. ExoProK: A Practical Method for the Isolation of Small Extracellular Vesicles from Pleural Effusions.

10. Selection and Evaluation of Tissue Specific Reference Genes in Lucilia sericata during an Immune Challenge.