Literature DB >> 24034322

Polysome analysis of mammalian cells.

Shan L He1, Rachel Green.   

Abstract

To assess the global translational level of mammalian cells (see similar protocols for bacteria and yeast on Analysis of polysomes from bacteria, Polysome Profile Analysis - Yeast and Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae).
© 2013 Elsevier Inc. All rights reserved.

Entities:  

Keywords:  Cell lysate; Genome-wide analysis techniques; Immobilize ribosomes; Mammalian cells; Polysome analysis; Size fractionation; Sucrose density gradient centrifugation

Mesh：

Substances：

Year:  2013        PMID： 24034322     DOI： 10.1016/B978-0-12-420037-1.00010-5

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  12 in total

1.  Cell-type specific polysome profiling from mammalian tissues.

Authors:  Joseph Seimetz; Waqar Arif; Sushant Bangru; Mikel Hernaez; Auinash Kalsotra
Journal:  Methods       Date:  2018-11-27       Impact factor: 3.608

2.  Deregulation of ribosomal protein expression and translation promotes breast cancer metastasis.

Authors:  Richard Y Ebright; Sooncheol Lee; Ben S Wittner; Kira L Niederhoffer; Benjamin T Nicholson; Aditya Bardia; Samuel Truesdell; Devon F Wiley; Benjamin Wesley; Selena Li; Andy Mai; Nicola Aceto; Nicole Vincent-Jordan; Annamaria Szabolcs; Brian Chirn; Johannes Kreuzer; Valentine Comaills; Mark Kalinich; Wilhelm Haas; David T Ting; Mehmet Toner; Shobha Vasudevan; Daniel A Haber; Shyamala Maheswaran; Douglas S Micalizzi
Journal:  Science       Date:  2020-02-06       Impact factor: 47.728

Review 3.  Selective footprinting of 40S and 80S ribosome subpopulations (Sel-TCP-seq) to study translation and its control.

Authors:  Susan Wagner; Jonathan Bohlen; Anna Herrmannova; Jan Jelínek; Thomas Preiss; Leoš Shivaya Valášek; Aurelio A Teleman
Journal:  Nat Protoc       Date:  2022-07-22       Impact factor: 17.021

4.  Accessing the Human Pluripotent Stem Cell Translatome by Polysome Profiling.

Authors:  Rubens Gomes-Júnior; Patrícia Shigunov; Bruno Dallagiovanna; Isabela Tiemy Pereira
Journal:  Methods Mol Biol       Date:  2022

5.  A Specialized Mechanism of Translation Mediated by FXR1a-Associated MicroRNP in Cellular Quiescence.

Authors:  Syed I A Bukhari; Samuel S Truesdell; Sooncheol Lee; Swapna Kollu; Anthony Classon; Myriam Boukhali; Esha Jain; Richard D Mortensen; Akiko Yanagiya; Ruslan I Sadreyev; Wilhelm Haas; Shobha Vasudevan
Journal:  Mol Cell       Date:  2016-03-03       Impact factor: 17.970

6.  Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion.

Authors:  Miroslava Stastna; Amandine Thomas; Juliana Germano; Somayeh Pourpirali; Jennifer E Van Eyk; Roberta A Gottlieb
Journal:  J Vis Exp       Date:  2018-08-29       Impact factor: 1.355

7.  Polysome Profiling without Gradient Makers or Fractionation Systems.

Authors:  Mack Sobhany; Robin E Stanley
Journal:  J Vis Exp       Date:  2021-06-01       Impact factor: 1.424

8.  Mechanisms of anti-vesicular stomatitis virus activity of deazaneplanocin and its 3-brominated analogs.

Authors:  Joyce Sweeney Gibbons; Sudip Khadka; Caroline G Williams; Lin Wang; Stewart W Schneller; Chong Liu; JoAnn M Tufariello; Christopher F Basler
Journal:  Antiviral Res       Date:  2021-05-19       Impact factor: 10.103

9.  Design of nucleotide-mimetic and non-nucleotide inhibitors of the translation initiation factor eIF4E: Synthesis, structural and functional characterisation.

Authors:  Fadi Soukarieh; Matthew W Nowicki; Amandine Bastide; Tuija Pöyry; Carolyn Jones; Kate Dudek; Geetanjali Patwardhan; François Meullenet; Neil J Oldham; Malcolm D Walkinshaw; Anne E Willis; Peter M Fischer
Journal:  Eur J Med Chem       Date:  2016-08-24       Impact factor: 6.514

10.  Transcription factor Hlx controls a systematic switch from white to brown fat through Prdm16-mediated co-activation.

Authors:  Lei Huang; Dongning Pan; Qingbo Chen; Lihua J Zhu; Jianhong Ou; Martin Wabitsch; Yong-Xu Wang
Journal:  Nat Commun       Date:  2017-07-12       Impact factor: 14.919
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