Literature DB >> 2403361

Addition of serum to electroporated cells enhances survival and transfection efficiency.

A B Bahnson1, S S Boggs.   

Abstract

Optimal electroporation efficiency of many cell types is associated with poor survival. We show that serum rapidly reseals the membranes of electroporated cells and that timely addition of serum following electroporation can improve cell survival and transfection efficiency.

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Year:  1990        PMID： 2403361     DOI： 10.1016/0006-291x(90)91210-j

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  9 in total

1.  Gene correction in hematopoietic progenitor cells by homologous recombination.

Authors:  S Hatada; K Nikkuni; S A Bentley; S Kirby; O Smithies
Journal:  Proc Natl Acad Sci U S A       Date:  2000-12-05       Impact factor: 11.205

2.  Optimization and evaluation of electroporation delivery of siRNA in the human leukemic CEM cell line.

Authors:  Anna Fyrberg; Kourosh Lotfi
Journal:  Cytotechnology       Date:  2010-10-19       Impact factor: 2.058

3.  Applications of electroporation of adherent cells in situ, on a partly conductive slide.

Authors:  L H Raptis; H L Brownell; S K Liu; K L Firth; L W MacKenzie; C D Stiles; J A Alberta
Journal:  Mol Biotechnol       Date:  1995-10       Impact factor: 2.695

4.  Efficient in situ electroporation of mammalian cells grown on microporous membranes.

Authors:  T A Yang; W C Heiser; J M Sedivy
Journal:  Nucleic Acids Res       Date:  1995-08-11       Impact factor: 16.971

Review 5.  Rat embryonic stem cells create new era in development of genetically manipulated rat models.

Authors:  Kazushi Kawaharada; Masaki Kawamata; Takahiro Ochiya
Journal:  World J Stem Cells       Date:  2015-08-26       Impact factor: 5.326

6.  Examination of gap junctional, intercellular communication by in situ electroporation on two co-planar indium-tin oxide electrodes.

Authors:  Aikaterini Anagnostopoulou; Jun Cao; Adina Vultur; Kevin Firth; Leda Raptis
Journal:  Mol Oncol       Date:  2007-06-20       Impact factor: 6.603

7.  Immobilization of electroporated cells for fabrication of cellular biosensors: physiological effects of the shape of calcium alginate matrices and foetal calf serum.

Authors:  Nikos Katsanakis; Andreas Katsivelis; Spiridon Kintzios
Journal:  Sensors (Basel)       Date:  2009-01-09       Impact factor: 3.576

Review 8.  Gene Electrotransfer: A Mechanistic Perspective.

Authors:  Christelle Rosazza; Sasa Haberl Meglic; Andreas Zumbusch; Marie-Pierre Rols; Damijan Miklavcic
Journal:  Curr Gene Ther       Date:  2016       Impact factor: 4.391

Review 9.  The Ins and Outs of Messenger RNA Electroporation for Physical Gene Delivery in Immune Cell-Based Therapy.

Authors:  Diana Campillo-Davo; Maxime De Laere; Gils Roex; Maarten Versteven; Donovan Flumens; Zwi N Berneman; Viggo F I Van Tendeloo; Sébastien Anguille; Eva Lion
Journal:  Pharmaceutics       Date:  2021-03-16       Impact factor: 6.321
  9 in total

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