Literature DB >> 24031907

Isolation of the first IMP-4 metallo-β-lactamase producing Klebsiella pneumoniae in Tianjin, China.

Jing Li1, Zhidong Hu, Qiaojuan Hu.   

Abstract

This study shows for the first time the mechanism of carbapenem resistance of a Klebsiella pneumoniae clinical isolate TJ8 recovered from Tianjin Medical University General Hospital ,China. The modified Hodge test and EDTA synergy test were performed for the screening of carbapenemases and metallo-β-lactamases (MBLs), respectively. Polymerase chain reactions and DNA sequencing confirmed that the strain carried IMP-4 metallo-β-lactamase (MBL) , SHV-11 and TEM-1 β-lactamase. Class I integron was positive and gave a 3.0-kb PCR amplicon .IMP-4 was located in Class I integron 5'CS. The gene determinants were organized in the order of bla IMP-4-orfII-orfIII.In all, the results show that IMP-4 MBL production caused the TJ8 resistance to carbapenems.

Entities:  

Keywords:  Klebsiella pneumoniae; integron; metallo-β-lactamases

Year:  2012        PMID： 24031907      PMCID： PMC3768900          DOI： 10.1590/S1517-838220120003000010

Source DB:  PubMed          Journal:  Braz J Microbiol        ISSN： 1517-8382            Impact factor:   2.476


  23 in total

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2.  Modified Hodge and EDTA-disk synergy tests to screen metallo-beta-lactamase-producing strains of Pseudomonas and Acinetobacter species.

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Journal:  Clin Microbiol Infect       Date:  2001-02       Impact factor: 8.067

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4.  Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR.

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5.  A novel family of potentially mobile DNA elements encoding site-specific gene-integration functions: integrons.

Authors:  H W Stokes; R M Hall
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8.  PCR typing of genetic determinants for metallo-beta-lactamases and integrases carried by gram-negative bacteria isolated in Japan, with focus on the class 3 integron.

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Authors:  M Edelstein; M Pimkin; I Palagin; I Edelstein; L Stratchounski
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Review 10.  Mobile gene cassettes and integrons: capture and spread of genes by site-specific recombination.

Authors:  R M Hall; C M Collis
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