Literature DB >> 24030197

Detection of salt bridges to lysines in solution in barnase.

Mike P Williamson1, Andrea M Hounslow, Joe Ford, Kyle Fowler, Max Hebditch, Poul Erik Hansen.   

Abstract

We show that salt bridges involving lysines can be detected by deuterium isotope effects on NMR chemical shifts of the sidechain amine. Lys27 in the ribonuclease barnase is salt bridged, and mutation of Arg69 to Lys retains a partially buried salt bridge. The salt bridges are functionally important.

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Year:  2013        PMID： 24030197     DOI： 10.1039/c3cc45602a

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  6 in total

1.  Effective strategy to assign ¹H- ¹⁵N heteronuclear correlation NMR signals from lysine side-chain NH3₃⁺ groups of proteins at low temperature.

Authors:  Alexandre Esadze; Levani Zandarashvili; Junji Iwahara
Journal:  J Biomol NMR       Date:  2014-08-17       Impact factor: 2.835

2.  Dynamic ion pair behavior stabilizes single α-helices in proteins.

Authors:  Matthew Batchelor; Marcin Wolny; Emily G Baker; Emanuele Paci; Arnout P Kalverda; Michelle Peckham
Journal:  J Biol Chem       Date:  2018-12-28       Impact factor: 5.157

Review 3.  Physicochemical Properties of Ion Pairs of Biological Macromolecules.

Authors:  Junji Iwahara; Alexandre Esadze; Levani Zandarashvili
Journal:  Biomolecules       Date:  2015-09-30

4.  A 13C-detected 15N double-quantum NMR experiment to probe arginine side-chain guanidinium 15Nη chemical shifts.

Authors:  Harold W Mackenzie; D Flemming Hansen
Journal:  J Biomol NMR       Date:  2017-11-10       Impact factor: 2.835

5.  How well do force fields capture the strength of salt bridges in proteins?

Authors:  Mustapha Carab Ahmed; Elena Papaleo; Kresten Lindorff-Larsen
Journal:  PeerJ       Date:  2018-06-11       Impact factor: 2.984

6.  The Structure of the "Vibration Hole" around an Isotopic Substitution-Implications for the Calculation of Nuclear Magnetic Resonance (NMR) Isotopic Shifts.

Authors:  Jürgen Gräfenstein
Journal:  Molecules       Date:  2020-06-24       Impact factor: 4.411
  6 in total

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