| Literature DB >> 24029944 |
Rogelio Zamilpa1, Jianhua Zhang, Ying Ann Chiao, Lisandra E de Castro Brás, Ganesh V Halade, Yonggang Ma, Sander O Hacker, Merry L Lindsey.
Abstract
Myocardial infarction (MI) is a leading cause of death worldwide. Permanent ligation of the left anterior descending coronary artery (LAD) is a commonly used surgical model to study post-MI effects in mice. LAD occlusion induces a robust wound healing response that includes extracellular matrix (ECM) remodeling. This chapter provides a detailed guide on the surgical procedure to permanently ligate the LAD. Additionally, we describe a prototype method to enrich cardiac tissue for ECM, which allows one to focus on ECM remodeling in the left ventricle following surgically induced MI in mice.Entities:
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Year: 2013 PMID： 24029944 PMCID： PMC3970183 DOI： 10.1007/978-1-62703-505-7_18
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745