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Literature DB >> 2402445

Molecular cloning and expression of four actin isoforms during Artemia development.

Abstract

Four cDNA clones coding for different Artemia actin isoforms have been isolated. Three of the clones contain the complete coding sequences while the fourth one lacks 145 bases, coding for the 49 amino terminal amino acids of the protein. The amino acid sequences predicted for the four actin isoforms identified are highly homologous to insect actins as well as to vertebrate cytoplasmic actins. The four identified cDNA clones code for mRNAs of 5.2, 1.9, 1.6 and 1.8 kb, respectively, whose expression is regulated during development. Three of the actin mRNAs are present in cryptobiotic embryos while the other is not. The steady-state levels of all four mRNAs increase during development to reach maximal levels by 10-15 hours of development and decrease thereafter. The total number of actin genes encoded in the Artemia genome has been estimated as 8 to 10 by Southern analysis of total DNA.

Entities: Disease

Mesh：

Substances：
Actins
DNA

Year: 1990 PMID： 2402445 PMCID： PMC332145 DOI： 10.1093/nar/18.17.5219

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

42 in total

1. Levels of translatable mRNAs for cell surface protein, collagen precursors, and two membrane proteins are altered in Rous sarcoma virus-transformed chick embryo fibroblasts.

Authors: S L Adams; M E Sobel; B H Howard; K Olden; K M Yamada; B de Crombrugghe; I Pastan
Journal: Proc Natl Acad Sci U S A Date: 1977-08 Impact factor: 11.205

Molecular cloning and expression of four actin isoforms during Artemia development.

1. Levels of translatable mRNAs for cell surface protein, collagen precursors, and two membrane proteins are altered in Rous sarcoma virus-transformed chick embryo fibroblasts.

2. Screening lambdagt recombinant clones by hybridization to single plaques in situ.

3. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

4. Restriction mapping of the rRNA genes from Artemia larvae.

5. The actin genes of Drosophila: protein coding regions are highly conserved but intron positions are not.

6. Plasmid screening at high colony density.

7. Multiple, heterogeneous actin genes in Dictyostelium.

8. Levels of the RNA polymerases during the early larval development of Artemia.

9. The actin genes of Drosophila: a dispersed multigene family.

10. Multiple actin-related sequences in the Drosophila melanogaster genome.

1. New nucleotide sequence data on the EMBL File Server.

2. Insect muscle actins differ distinctly from invertebrate and vertebrate cytoplasmic actins.

3. A TaqI RFLP for the human arylsulfatase A gene.

4. Actin genes and their expression in pacific white shrimp, Litopenaeus vannamei.

5. Profiles of nuclear and mitochondrial encoded mRNAs in developing and quiescent embryos of Artemia franciscana.

6. Actin gene structure in two Artemia species, A. franciscana and A. parthenogenetica.