OBJECTIVES: To identify the active principles, determine the anti-diabetes activity of fraction of Stereospermum tetragonum root. MATERIALS AND METHODS: The efficacy was evaluated in streptozotocin induced type 2 diabetic rats and the anti-hyperglycemic activity was studied by glucose tolerance test. The major active compounds were isolated by solvent fractionation and chromatographic techniques and characterized with spectral data. RESULTS: The active fraction of S. tetragonum showed presence of anti-diabetes mellitus activity in type-2 diabetic rats. It did not significantly influence insulin release from cultured islets. Two active principles (active at 2 mg/kg dose) were isolated and characterized with spectral data. One of them was identified as an iridoid type glycoside and the other one was a lapachol like compound (derivative of naphthoquinone). CONCLUSIONS: Two active principles from the anti-diabetes fraction of S. tetragonum root were isolated and identified as an iridoid glycoside and a naphthoquinone derivative.
OBJECTIVES: To identify the active principles, determine the anti-diabetes activity of fraction of Stereospermum tetragonum root. MATERIALS AND METHODS: The efficacy was evaluated in streptozotocin induced type 2 diabeticrats and the anti-hyperglycemic activity was studied by glucose tolerance test. The major active compounds were isolated by solvent fractionation and chromatographic techniques and characterized with spectral data. RESULTS: The active fraction of S. tetragonum showed presence of anti-diabetes mellitus activity in type-2 diabeticrats. It did not significantly influence insulin release from cultured islets. Two active principles (active at 2 mg/kg dose) were isolated and characterized with spectral data. One of them was identified as an iridoid type glycoside and the other one was a lapachol like compound (derivative of naphthoquinone). CONCLUSIONS: Two active principles from the anti-diabetes fraction of S. tetragonum root were isolated and identified as an iridoid glycoside and a naphthoquinone derivative.
Authors: Marcela Brissova; Masakazu Shiota; Wendell E Nicholson; Maureen Gannon; Susan M Knobel; David W Piston; Christopher V E Wright; Alvin C Powers Journal: J Biol Chem Date: 2002-01-07 Impact factor: 5.157