| Literature DB >> 240233 |
U W Schaefer, C G Schmidt, K A Dicke, D W van Bekkum, G Schmitt.
Abstract
The CFU-S potential of mouse bone marrow, the transplantation capacity of autologous and allogeneic monkey bone marrow and the CFU-C potential of human bone marrow can be cryopreserved without loss of viability. It is critical for an optimal stem cell recovery to avoid an osmotic shock after thawing. The intracellular cryoprotectives glycerol and DMSO which provide a high osmotic pressure should be removed from the thawed cells by a slow stepwise dilution procedure.Entities:
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Year: 1975 PMID: 240233 DOI: 10.1007/bf00573015
Source DB: PubMed Journal: Z Krebsforsch Klin Onkol Cancer Res Clin Oncol ISSN: 0084-5353