| Literature DB >> 24021647 |
Shalini Sethurathinam1, Laishram Pradeepkumar Singh, Porkodi Panneerselvam, Bernadette Byrne, Jeak Ling Ding.
Abstract
Apoptosis is a vital defense mechanism for the clearance of infected cells. Ubiquitously expressed transcript (UXT), which exists in two isoforms (V1 and V2), interact with both apoptotic and cellular proteins. By yeast two-hybrid analysis, we found that UXT interacts with SARM (sterile α and HEAT armadillo motif-containing protein). Since SARM is a TLR adaptor which induces intrinsic apoptosis following immune activation, we were prompted to query whether UXT and SARM might co-regulate apoptosis. We found that the UXT isoforms elicit dual opposing regulatory effects on SARM-induced apoptosis; while UXT V1, co-expressed with SARM, caused a reduction in caspase 8 activity, UXT V2 strongly increased caspase 8 activity and enhanced SARM-induced apoptosis by activating the extrinsic pathway and depolarizing the mitochondria.Entities:
Keywords: Apoptosis; FADD; FAS associated protein with death domain; Infection-inflammation condition; LPS; MAVS; Mitochondrial depolarization; SARM; Sterile α and HEAT armadillo motif-containing protein (SARM); TIR; TLR; TMRE; TNF; TNF receptor associated death domain; TNF receptor–associated factor 2; TRADD; TRAF2; UXT; Ubiquitously expressed transcript (UXT); lipopolysaccharide; mitochondrial anti-viral signaling protein; sterile α and heat armadillo motif-containing protein; tetramethylrhodamine ethyl ester perchlorate; toll-like receptor; toll/interleukin-1 receptor; tumor necrosis factor; ubiquitously expressed transcript
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Year: 2013 PMID： 24021647 DOI： 10.1016/j.febslet.2013.08.033
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124