Yu Xiao1, Tinchiu Li, Enlan Xia, Xiuli Yang, Xiao Sun, Yingfang Zhou. 1. Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing 100038, People's Republic of China; Department of Obstetrics and Gynaecology, Jessop Wing, Sheffield Teaching Hospitals and Sheffield Hallam University, Sheffield, S10 2SF, United Kingdom; Hysterosopic Center, Fuxing Hospital, Capital Medical University, Beijing 100038, People's Republic of China.
Abstract
OBJECTIVE: To investigate the expression of integrin β3 and osteopontin (OPN) in the eutopic endometrium of adenomyosis and to characterize possible endometrial defects in adenomyosis. STUDY DESIGN: Twenty-eight adenomyosis patients and 27 control fertile women were involved in this study. Endometrial samples were collected during the implantation window. Integrin β3 subunits and OPN in the adenomyosis and control endometria were assessed by immunohistochemical staining and quantitative real-time polymerase chain reaction. A one-tailed t-test and a non-parametric Mann-Whitney U-test were used to test significance. RESULTS: The mRNA and immunostaining intensity of integrin β3 and OPN were significantly lower in the adenomyosis patients than in the controls. CONCLUSIONS: Abnormal expression of integrin β3 and OPN in the endometrium of adenomyosis may contribute to infertility in some patients.
OBJECTIVE: To investigate the expression of integrin β3 and osteopontin (OPN) in the eutopic endometrium of adenomyosis and to characterize possible endometrial defects in adenomyosis. STUDY DESIGN: Twenty-eight adenomyosispatients and 27 control fertile women were involved in this study. Endometrial samples were collected during the implantation window. Integrin β3 subunits and OPN in the adenomyosis and control endometria were assessed by immunohistochemical staining and quantitative real-time polymerase chain reaction. A one-tailed t-test and a non-parametric Mann-Whitney U-test were used to test significance. RESULTS: The mRNA and immunostaining intensity of integrin β3 and OPN were significantly lower in the adenomyosispatients than in the controls. CONCLUSIONS: Abnormal expression of integrin β3 and OPN in the endometrium of adenomyosis may contribute to infertility in some patients.
Authors: Amrita Singh; Carmine Fedele; Huimin Lu; Marja T Nevalainen; James H Keen; Lucia R Languino Journal: Mol Cancer Res Date: 2016-07-20 Impact factor: 5.852