| Literature DB >> 24014405 |
Lei Zhong1, Barbara Martinez-Pastor1, Dafne M Silberman1, Carlos Sebastian1, Raul Mostoslavsky1.
Abstract
Most of the sirtuins' nuclear substrates identified so far are histones or other chromatin-associated proteins and, thus, it is of special relevance the development of good biochemical techniques to analyze the biology of these proteins in the context of chromatin. Here, we describe several of the chromatin-based techniques to identify sirtuins' substrates, including a chromatin immunoprecipitation (ChIP) protocol, an acid-extraction protocol, and a nucleosomal immunoprecipitation protocol to analyze putative sirtuin chromatin interactors.Entities:
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Year: 2013 PMID： 24014405 PMCID： PMC4605221 DOI： 10.1007/978-1-62703-637-5_10
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745